Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile women due to tubal problems. A total of 208 infertile women due to tubal problems underwent IVF-ET treatment. The women were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation. Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group. LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile women with tubal problems that using single blastocyst transfer method.

中文翻译：

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胚胎培养基中的 LIF 是输卵管阻塞患者 IVF-ET 后临床妊娠的预测标志物。

白血病抑制因子 (LIF) 在一系列生殖事件中发挥了至关重要的作用，包括卵泡生长、胚胎生长和分化。然而，胚胎培养基中的 LIF 水平是否可以作为临床妊娠的标志尚不清楚。在这项研究中，我们旨在调查胚胎培养基中的 LIF 水平是否可以作为因输卵管问题而导致的不孕女性体外受精 - 胚胎移植 (IVF-ET) 妊娠结局的预测标志物。共有 208 名因输卵管问题导致的不孕妇女接受了 IVF-ET 治疗。根据临床上是否怀孕，这些妇女被分为两组。测定胚胎培养基中LIF水平，分析LIF水平与胚胎质量和临床妊娠结局的相关性。在囊胚移植当天收集胚胎培养基。与非妊娠组相比，妊娠组囊胚移植当天胚胎培养基中的LIF水平显着升高。胚胎培养基中的 LIF 水平可用作非侵入性辅助生物标志物，用于预测使用单囊胚移植方法存在输卵管问题的不孕妇女的临床妊娠。