The ultimate goal of pancreatic tissue engineering is to create a long-lived substitute organ to treat diabetes. However, the lack of neovascularization and the occurrence of immune response limit the efficacy of tissue-engineered pancreas after in vivo transplantation. Platelet-rich plasma (PRP) is an autologous platelet concentrate containing a large number of growth factors and immunoregulatory factors. The aim of this study was to evaluate rat pancreatic decellularized scaffold (PDS) loaded with PRP for vascularization, host inflammatory response and macrophage polarization in an animal model. The study results indicated that compared to PDS, PRP-loading PDS exhibited the enhanced mechanical properties and released growth factors in a slow and sustained manner to supplement the loss of growth factors during decellularization. In vitro, human umbilical vein endothelial cells (HUVECs) were seeded in PDS and PRP-loading PDS, and cultured in the circular perfusion system. When compared with PDS, PRP-loading PDS significantly promoted the colonization, proliferation and pro-angiogenic genes expression of cells on scaffolds. In vivo, PDS loaded with PRP then re-endothelialized with HUVECs were implanted subcutaneously in rats, which enhanced the angiogenesis of scaffolds, inhibited the host inflammatory response, and induced the polarization dominated by pro-regenerative M2 macrophages that also facilitated tissue vascular regeneration. Thus, the re-endothelialized PRP-loading PDS may represent a promising bioengineered pancreas with sustained vascularization and excellent biocompatibility.

胰腺组织工程的最终目标是创造一种长寿的替代器官来治疗糖尿病。然而，缺乏新生血管和免疫反应的发生限制了组织工程胰腺在体内后的疗效。移植。富血小板血浆（PRP）是一种含有大量生长因子和免疫调节因子的自体血小板浓缩物。本研究的目的是评估负载 PRP 的大鼠胰腺脱细胞支架 (PDS) 在动物模型中的血管化、宿主炎症反应和巨噬细胞极化。研究结果表明，与PDS相比，负载PRP的PDS表现出增强的机械性能，并以缓慢而持续的方式释放生长因子，以补充脱细胞过程中生长因子的损失。在体外，人脐静脉内皮细胞 (HUVEC) 被接种在 PDS 和 PRP 负载 PDS 中，并在循环灌注系统中培养。与PDS相比，加载PRP的PDS显着促进了定植，支架上细胞的增殖和促血管生成基因表达。在体内，载有 PRP 的 PDS 然后用 HUVEC 重新内皮化植入大鼠皮下，这增强了支架的血管生成，抑制了宿主炎症反应，并诱导了由促再生 M2 巨噬细胞主导的极化，也促进了组织血管再生。因此，再内皮化的 PRP 负载 PDS 可能代表具有持续血管化和优异生物相容性的有前途的生物工程胰腺。并诱导由促再生 M2 巨噬细胞主导的极化，这也促进了组织血管再生。因此，再内皮化的 PRP 负载 PDS 可能代表具有持续血管化和优异生物相容性的有前途的生物工程胰腺。并诱导由促再生 M2 巨噬细胞主导的极化，这也促进了组织血管再生。因此，再内皮化的 PRP 负载 PDS 可能代表具有持续血管化和优异生物相容性的有前途的生物工程胰腺。