One of the most common prophylactic techniques to solve prosthetic joint infection (PJI) is incorporation of antibiotics into acrylic bone cement to prevent bacterial colonization and proliferation by providing local antibiotic delivery directly at the implant site. Further, there has been a significant concern over the efficacy of commonly used antibiotics within bone cement due to the rise in multi-drug resistant (MDR) microorganisms. Selenium is an essential trace element that has multiple beneficial effects for human health and its chemotherapeutic action is well known. It was reported that nanostructured selenium enhanced bone cell adhesion and has an increased osteoblast function. In this context, we used the selenium nanoparticles (SeNPs) to improve antibacterial and antioxidant properties of poly (methyl methacrylate) (PMMA) and tri calcium phosphate (TCP)-based bone cements, and to reduce of the infection risk caused by orthopedic implants. As another novelty of this study, we proposed phosphatidylcholine (PC) as a unique and natural stabilizer in the synthesis of selenium nanoparticles. After the structural analysis of the prepared bone cements was performed, in vitro osteointegration and antibacterial efficiency were tested using MC3T-E1 (mouse osteoblastic cell line) and SaOS-2 (human primary osteogenic sarcoma) cell lines, and S. aureus (Gram positive) and E.coli (Gram negative) strains, respectively. More importantly, PC-SeNPs-reinforced bone cements exhibited significant effect against E. coli, compared to S. aureus and a dose-dependent antibacterial activity against both bacterial strains tested. Meanwhile, these bone cements induced the apoptosis of SaOS-2 through increased reactive oxygen species without negatively influencing the viability of the healthy cell line. Furthermore, the obtained confocal images revealed that PC-SeNPs (103.7 ± 0.56 nm) altered the cytoskeletal structure of SaOS-2 owing to SeNPs-induced apoptosis, when MC3T3-E1 cells showed a typical spindle-shaped morphology. Taken together, these results highlighted the potential of PC-SeNPs-doped bone cements as an effective graft material in bone applications.

解决假体关节感染 (PJI) 的最常见预防技术之一是将抗生素掺入丙烯酸骨水泥中，通过直接在植入部位提供局部抗生素来防止细菌定植和增殖。此外，由于耐多药 (MDR) 微生物的增加，骨水泥中常用抗生素的功效引起了极大的关注。硒是一种必需的微量元素，对人类健康具有多种有益作用，其化学治疗作用是众所周知的。据报道，纳米结构的硒增强了骨细胞的粘附并具有增强的成骨细胞功能。在这种情况下，我们使用硒纳米颗粒 (SeNPs) 来提高聚甲基丙烯酸甲酯 (PMMA) 和磷酸三钙 (TCP) 基骨水泥的抗菌和抗氧化性能，并降低骨科植入物引起的感染风险。作为本研究的另一个新颖之处，我们提出了磷脂酰胆碱 (PC) 作为合成硒纳米颗粒的独特天然稳定剂。在对制备的骨水泥进行结构分析后，使用 MC3T-E1（小鼠成骨细胞系）和 SaOS-2（人原代成骨肉瘤）细胞系测试体外骨整合和抗菌效率，以及 我们建议磷脂酰胆碱 (PC) 作为一种独特的天然稳定剂，用于合成硒纳米颗粒。在对制备的骨水泥进行结构分析后，使用 MC3T-E1（小鼠成骨细胞系）和 SaOS-2（人原代成骨肉瘤）细胞系测试体外骨整合和抗菌效率，以及 我们建议磷脂酰胆碱 (PC) 作为一种独特的天然稳定剂，用于合成硒纳米颗粒。在对制备的骨水泥进行结构分析后，使用 MC3T-E1（小鼠成骨细胞系）和 SaOS-2（人原代成骨肉瘤）细胞系测试体外骨整合和抗菌效率，以及分别是金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）菌株。更重要的是，与金黄色葡萄球菌相比，PC-SeNPs 增强的骨水泥对大肠杆菌表现出显着的效果以及对测试的两种细菌菌株的剂量依赖性抗菌活性。同时，这些骨水泥通过增加活性氧诱导 SaOS-2 细胞凋亡，而不会对健康细胞系的生存能力产生负面影响。此外，获得的共聚焦图像显示 PC-SeNPs (103.7 ± 0.56 nm) 由于 SeNPs 诱导的细胞凋亡而改变了 SaOS-2 的细胞骨架结构，当 MC3T3-E1 细胞显示出典型的纺锤形形态时。总之，这些结果突出了 PC-SeNPs 掺杂的骨水泥作为骨应用中有效移植材料的潜力。