Enantiomeric resolution and molecular docking studies of meclizine hydrochloride on polysaccharide‐based chiral stationary phase comprising cellulose tris(4‐methylbenzoate) chiral selector (150 × 4.6 mm, 3.0 μm) were presented. The mobile phase used was acetonitrile:10mM ammonium bicarbonate (95:05, v/v). The developed technique was used to perform the enantioselective assay of meclizine hydrochloride in its marketed formulation. The elution order of meclizine hydrochloride enantiomers was determined by docking studies. Target compound was extracted from rabbit plasma using protein precipitation technique, followed by development of bioanalytical chiral separation method using the same matrix. Application of the method to determine pharmacokinetic parameters of meclizine hydrochloride enantiomers was performed using Phoenix WinNonlin 8.1 software. The results demonstrated stereoselective disposition of meclizine hydrochloride enantiomers in rabbits.

中文翻译：

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生物分析手性色谱技术和盐酸对氯苯甲嗪对映选择性分离的对接研究：在兔体内药代动力学研究中的应用。

提出了盐酸美克利嗪在包含纤维素三（4-甲基苯甲酸酯）手性选择剂（150×4.6 mm，3.0μm）的基于多糖的手性固定相上的对映体拆分和分子对接研究。所使用的流动相是乙腈：10mM碳酸氢铵（95：05，v / v）。所开发的技术用于以市售配方进行盐酸美克利嗪的对映选择性测定。通过对接研究确定盐酸美克利嗪对映体的洗脱顺序。使用蛋白质沉淀技术从兔血浆中提取目标化合物，然后开发使用相同基质的生物分析手性分离方法。使用Phoenix WinNonlin 8.1软件进行该方法测定盐酸美克利嗪对映体的药代动力学参数的应用。