Background: Salinomycin, an ionophore antibiotic, has a strong anti-cancer effect, inducing the apoptosis of cancer cells and cancer stem cells.

Objective: The aim of the study was to assess the influence of salinomycin on the expression profile of genes related to stemness and miRNA regulating their expression in endometrial cancer cells.

Methods: Endometrial cancer cells of cell line Ishikawa were exposed to salinomycin at concentrations in the range of 0.1-100 μM, with the aim of determining its pro-apoptotic potential and the concentration which would cause the death of 50% of the cells (Sulforhodamine B test). In the following stages, the cells were incubated with the drug at a concentration of 1μM for 12,24 and 48 hour periods and compared to the control. Determining the changes in the expression of the genes related to stemness and regulating their miRNA was done using the microarray technique and RTqPCR. ELISA assay was performed in order to determine the level of TGFβ2, COL14A1, CDH2, WNT5A in cell culture under salinomycin treatment in comparison to the control.

Results: Salinomycin caused the apoptosis of cells. For the concentration of 0.1 μM, a decrease in the population of living cells by 11.9% was determined. For 1 μM, it was 49.8%, for 10 μM -69.4%, and for a concentration of 100 μM - 87.9%. The most noticeable changes in the expression caused by the addition of salinomycin into the culture were noted for mRNA: TGFβ2; WNT5A (up-regulated); COL14A1; CDH2 (down-regulated), as well as miRNA: hsa-miR-411 (up-regulated); hsa-miR-200a; hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-374; hsa-miR-374b (down-regulated).

Conclusion: It was confirmed that salinomycin has an influence on the stemness process. The most noticeable changes in the expression were noted for mRNA: TGFβ2; COL14A1; CDH2; WNT5A, as well as for miRNA: hsa-miR-200a; hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-411; hsa-miR- 374a; hsa-miR-374b.

背景：盐霉素，一种离子载体抗生素，具有很强的抗癌作用，可诱导癌细胞和癌细胞干细胞的凋亡。

目的：研究目的是评估沙利霉素对子宫内膜癌细胞中与干性相关的基因的表达谱和调控其表达的miRNA的影响。

方法：以0.1-100μM的浓度将石川细胞系的子宫内膜癌细胞暴露于沙利霉素中，以测定其促凋亡潜力以及可导致50％细胞死亡的浓度（磺胺丁二胺B测试）。在接下来的阶段中，将细胞与浓度为1μM的药物一起孵育12、24和48小时，并与对照进行比较。使用微阵列技术和RTqPCR确定与干性相关的基因的表达变化并调节其miRNA。为了确定与对照相比，在沙利霉素处理下细胞培养物中TGFβ2，COL14A1，CDH2，WNT5A的水平，进行了ELISA分析。

结果：沙利霉素引起细胞凋亡。对于0.1μM的浓度，确定活细胞数量减少了11.9％。对于1μM，它是49.8％，对于10μM-69.4％，对于100μM-87.9％的浓度。对于mRNA：TGFβ2；在培养基中添加沙利霉素引起的表达变化最明显。WNT5A（上调）；COL14A1；CDH2（下调），以及miRNA：hsa-miR-411（上调）；hsa-miR-200a; hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-374; hsa-miR-374b（下调）。

结论：证实了盐霉素对茎干过程有影响。对于mRNA：TGFβ2；表达最明显的变化。COL14A1；CDH2; WNT5A，以及miRNA：hsa-miR-200a；hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-411; hsa-miR-374a; hsa-miR-374b。