The objective of this work was to evaluate the effectiveness of the putative biocontrol agents (PBA) Bacillus paralicheniformis and Trichoderma asperelloides in vitro and in vivo to control two of the most important tomato plant diseases: vascular wilt (Fusarium oxysporum) and early blight (Alternaria alternata). The assessment of the in vitro interactions between the PBA and the phytopathogenic fungi was performed by dual confrontation assays. The biocontrol effectiveness of the individual and combined PBA treatments towards individual phytopathogen inoculations was evaluated in tomato plants. T. asperelloides was able to exert an outstanding mycoparasitic effect on both phytopathogenic fungi in the in vitro tests by hyphal strangulation and penetration. In addition, the individual PBA treatments were effective in the biocontrol of A. alternata and F. oxysporum in tomato plants reducing the plant disease severity in more than 53.8 and 66.7% for each of the pathogens, respectively. On the other hand, the combined use of the tested strains showed similar effectiveness in the biocontrol of A. alternata, but no synergism was observed. In addition, it was concluded that B. paralicheniformis protected the plants from the attack of A. alternata through the induction of the systemic resistance of the plant. This study demonstrated the effectiveness of the individual and combined use of the strains tested for the biocontrol of A. alternata and F. oxysporum in tomato plants.

这项工作的目的是在体外和体内评估假定的生物防治剂（PBA）副芽孢杆菌和曲霉木霉菌对两种最重要的番茄植物病的防治效果：血管枯萎病（Fusarium oxysporum）和早疫病（Alternaria）交替））。通过双重对抗试验对PBA和植物致病真菌之间的体外相互作用进行评估。在番茄植物中评估了单独的和联合的PBA处理对单独的植物病原菌接种的生物防治效力。T. asperelloides在体外测试中，通过菌丝扼杀和穿透，能够对植物致病真菌均表现出出色的霉菌寄生作用。另外，个别PBA治疗是有效的生物控制链格孢和尖孢镰孢番茄植物减少植物病害严重程度超过53.8和66.7％的每个的病原体，分别。在另一方面，组合使用的测试菌株的表现出的生物控制效力类似A.孢，但没有观察到协同作用。此外，得出的结论是，副粘芽孢杆菌可保护植物免受交链孢霉的侵袭。通过诱导植物的系统抗性。这项研究表明单独的和组合使用的用于生物防治测试的菌株的有效性链格孢和尖孢镰孢番茄植物。