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Transglutaminase-mediated assembly of multi-enzyme pathway onto TMV brush surfaces for synthesis of bacterial autoinducer-2.
Biofabrication ( IF 8.2 ) Pub Date : 2020-07-28 , DOI: 10.1088/1758-5090/ab9e7a
Narendranath Bhokisham 1 , Yi Liu , Adam D Brown , Gregory F Payne , James N Culver , William E Bentley
Affiliation  

Bioelectronic microdevices, with spatially arranged biosynthetic machinery, can be programmed to convert raw materials to high-value products in a controlled manner. Generic methods for biofunctionalization that enable precise control over biocomponent assembly at the nano and meso scales are necessary to diversify the range and capabilities of these systems. Here, we used tobacco mosaic virus (TMV) derived virus like particles (VLPs) as 3D interfacial scaffolds for the assembly of biosynthetic enzymes onto gold electrodes. The TMV capsids are aligned in a vertical brush configuration by cysteine modifications to the capsid protein and by taking advantage of the well-known gold/cysteine affinity. This alignment enables high surface density and biosynthetic enzyme-enzyme proximity. Enzymes are covalently tethered to the capsid protein of TMV by the N- and C-terminal addition of lysine-rich assembly domains which react with surface exposed glutamine residues on the capsid surfaces...

中文翻译:

转谷氨酰胺酶介导的多酶途径在TMV刷表面上的组装,用于合成细菌autoinducer-2。

具有空间排列的生物合成机械的生物电子微型设备可以进行编程,以受控方式将原材料转化为高价值产品。为了使这些系统的范围和功能多样化,需要能够在纳米和中尺度上精确控制生物组分组装的通用生物功能化方法。在这里,我们使用烟草花叶病毒(TMV)衍生的病毒样颗粒(VLP)作为3D界面支架,将生物合成酶组装到金电极上。通过对衣壳蛋白进行半胱氨酸修饰并利用众所周知的金/半胱氨酸亲和力,将TMV衣壳以垂直刷状排列。这种比对可以实现高表面密度和生物合成酶与酶的接近度。
更新日期:2020-07-30
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