Protists provide insights into the diversity and function of RNA viruses in marine systems. Among them, marine macroalgae are good targets for RNA virome analyses because they have a sufficient biomass in nature. However, RNA viruses in macroalgae have not yet been examined in detail, and only partial genome sequences have been reported for the majority of RNA viruses. Therefore, to obtain further insights into the distribution and diversity of RNA viruses associated with marine protists, we herein examined RNA viruses in macroalgae and a diatom. We report the putative complete genome sequences of six novel RNA viruses from two marine macroalgae and one diatom holobiont. Four viruses were not classified into established viral genera or families. Furthermore, a virus classified into Totiviridae showed a genome structure that has not yet been reported in this family. These results suggest that a number of distinct RNA viruses are widespread in a broad range of protists.

中文翻译：

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从海洋大型藻类和硅藻中鉴定的病毒 RNA 基因组

原生生物提供了对海洋系统中 RNA 病毒的多样性和功能的见解。其中，海洋大型藻类是 RNA 病毒组分析的良好目标，因为它们在自然界中具有足够的生物量。然而，尚未对大型藻类中的 RNA 病毒进行详细研究，大多数 RNA 病毒仅报道了部分基因组序列。因此，为了进一步了解与海洋原生生物相关的 RNA 病毒的分布和多样性，我们在此检查了大型藻类和硅藻中的 RNA 病毒。我们报告了来自两种海洋大型藻类和一种硅藻全息生物的六种新型 RNA 病毒的假定完整基因组序列。四种病毒未归入已建立的病毒属或科。此外，一种归类为 Totiviridae 的病毒显示了该科尚未报道的基因组结构。这些结果表明，许多不同的 RNA 病毒在广泛的原生生物中广泛分布。