Melatonin (MT) regulates several physiological activities in plants. However, information on how MT regulates soybean growth under low-temperature (LT) stress is lacking. To better understand how MT promotes plant growth and development under LT stress, we designed this study to evaluate the role of MT pretreatment on soybean seedlings exposed to LT stress. Our results showed that LT stress increased oxidative damage by increasing reactive oxygen species (ROS) accumulation, which affected the growth and development of soybean seedlings. However, the application of 5 µmol L–1 MT significantly decreased the oxidative damage by increasing plant mineral element concentrations and the transcript abundance of antioxidant related genes, which enhanced the decrease in ROS accumulation. These results collectively suggest the involvement of MT in improving LT stress tolerance of soybean seedlings by mediating plant mineral elements and the expression of genes involved in the antioxidant pathway.

中文翻译：

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褪黑激素通过介导参与抗氧化途径的植物矿物质元素和基因促进大豆幼苗免受低温胁迫的生长的证据


褪黑激素 (MT) 调节植物的多种生理活动。然而，关于 MT 如何在低温 (LT) 胁迫下调节大豆生长的信息尚缺乏。为了更好地了解 MT 如何在 LT 胁迫下促进植物生长发育，我们设计了本研究来评估 MT 预处理对暴露于 LT 胁迫的大豆幼苗的作用。我们的结果表明，LT胁迫通过增加活性氧（ROS）积累来增加氧化损伤，从而影响大豆幼苗的生长和发育。然而，5 µmol·L-1 MT的应用通过增加植物矿物质元素浓度和抗氧化相关基因的转录本丰度，显着减少氧化损伤，从而增强ROS积累的减少。这些结果共同表明MT通过介导植物矿物质元素和抗氧化途径相关基因的表达来提高大豆幼苗的LT胁迫耐受性。