DNA damage signals transducer RING finger protein 8 (RNF8) is involved in maintaining genomic stability by facilitating the repair of DNA double-strand breaks (DSB) via ubiquitin signaling. By analyzing the TCGA database and colon cancer tissue microarrays, we found that the expression level of RNF8 was positively correlated with that of c-Myc in colon cancer, which were closely associated with poor survival of colon cancer patients. Furthermore, overexpressing and knocking down RNF8 increased and decreased the expression of c-Myc in colon cancer cells, respectively. In addition, RNF8 interacted with β-catenin and facilitated its nuclear translocation by conjugating K63 polyubiquitination on it. These observations suggested a de novo role of RNF8 in promoting the progression of colon cancer by inducing β-catenin-mediated c-Myc expression.

中文翻译：

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RNF8 诱导 β-catenin 介导的 c-Myc 表达并促进结肠癌增殖。

DNA 损伤信号转导环指蛋白 8 (RNF8) 通过泛素信号传导促进 DNA 双链断裂 (DSB) 的修复，从而参与维持基因组稳定性。通过对TCGA数据库和结肠癌组织芯片的分析，我们发现结肠癌中RNF8的表达水平与c-Myc的表达水平呈正相关，这与结肠癌患者的生存率差密切相关。此外，过表达和敲低 RNF8 分别增加和降低了结肠癌细胞中 c-Myc 的表达。此外，RNF8 与 β-catenin 相互作用并通过在其上结合 K63 多聚泛素化促进其核易位。这些观察结果表明 RNF8 通过诱导 β-连环蛋白介导的 c-Myc 表达在促进结肠癌进展中发挥了从头作用。