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MicroRNA-527 Induces Proliferation and Cell Cycle in Esophageal Squamous Cell Carcinoma Cells by Repressing PH Domain Leucine-Rich-Repeats Protein Phosphatase 2.
Dose-Response ( IF 2.3 ) Pub Date : 2020-05-22 , DOI: 10.1177/1559325820928687
Qingjie Xian 1 , Ronglei Zhao 2 , Juanjuan Fu 1
Affiliation  

Increasing evidence indicated that microRNAs served dominant roles in carcinogenesis and cancer progression by targeting potential downstream genes. In our study, we found that miR-527 was an upregulated expression in human esophageal squamous cell carcinoma (ESCC) cells and tissues. Furthermore, overexpression of miR-527 promoted cell proliferation and colony formation, enhanced anchorage-independent growth ability, and contributed to cell cycle. In addition, protein phosphatase 2 (PHLPP2) was identified as the direct downstream target gene of miR-527 and was confirmed by luciferase gene reporter assay. In summary, we concluded that miR-527 acted as an oncogenic microRNA in ESCC development by directly targeting PHLPP2 might be a novel therapeutic target for the treatment of ESCC.



中文翻译:

MicroRNA-527通过抑制PH结构域亮氨酸重复蛋白磷酸酶2诱导食管鳞状细胞癌细胞的增殖和细胞周期。

越来越多的证据表明,microRNA通过靶向潜在的下游基因在癌变和癌症进展中起着主导作用。在我们的研究中,我们发现miR-527在人食道鳞状细胞癌(ESCC)细胞和组织中表达上调。此外,miR-527的过表达促进细胞增殖和集落形成,增强锚定非依赖性生长能力,并有助于细胞周期。此外,蛋白磷酸酶2(PHLPP2)被鉴定为miR-527的直接下游靶基因,并通过荧光素酶基因报告基因检测法得到证实。总而言之,我们得出的结论是,miR-527通过直接靶向PHLPP2在ESCC发育中充当致癌微RNA可能是ESCC治疗的新型治疗靶标。

更新日期:2020-06-30
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