Surgical resection in tongue cancer can impair speech and swallowing, reducing quality of life. There is a need for biomaterials that can regenerate tongue muscle tissue defects. Ideally, such a biomaterial would allow controlled release of therapeutic proteins, support the survival and differentiation of therapeutic cells, and promote tongue muscle regeneration in vivo. The aim of the current study was to assess these factors in an acryloyl group-modified crosslinked nanogel, consisting of cholesterol-bearing pullulan hydrogel nanoparticles, to determine its potential as a regenerative therapeutic following tongue resection. The hydrogel demonstrated substantial porosity and underwent slow biodegradation. When loaded with a model protein, the gel enabled sustained protein release over two weeks in serum, with no initial burst release. Mouse myoblasts demonstrated adhesion to the hydrogel and cell survival was observed up to one week. Gel-encapsulated myoblasts demonstrated normal myotube differentiation. Myoblast-loaded gels were implanted in a tongue defect in mice, and there was a significant increase in newly-regenerated myofibers in gel-implanted animals. The developed biomaterial platform demonstrates significant potential as a regenerative treatment following tongue resection, as it facilitates both protein and cell-mediated therapy, and stimulates tongue muscle regeneration in vivo.

中文翻译：

---

基于交联纳米凝胶的多孔水凝胶作为舌肌再生的功能支架。

舌癌手术切除会影响语言和吞咽功能，降低生活质量。需要能够再生舌头肌肉组织缺陷的生物材料。理想情况下，这种生物材料将允许治疗性蛋白质的受控释放，支持治疗性细胞的存活和分化，并促进体内舌肌再生。本研究的目的是评估丙烯酰基修饰的交联纳米凝胶中的这些因素，该凝胶由含胆固醇的支链淀粉水凝胶纳米颗粒组成，以确定其作为舌切除后再生治疗剂的潜力。水凝胶显示出大量孔隙并经历缓慢的生物降解。当加载模型蛋白时，凝胶能够在血清中持续释放蛋白质超过两周，没有初始突释。小鼠成肌细胞表现出对水凝胶的粘附，并且观察到细胞存活长达 1 周。凝胶包裹的成肌细胞表现出正常的肌管分化。将载有成肌细胞的凝胶植入小鼠的舌头缺损处，并且在凝胶植入的动物中新再生的肌纤维显着增加。开发的生物材料平台显示出作为舌切除后再生治疗的巨大潜力，因为它有利于蛋白质和细胞介导的治疗，并在体内刺激舌肌再生。并且在凝胶植入的动物中新再生的肌纤维显着增加。开发的生物材料平台显示出作为舌切除后再生治疗的巨大潜力，因为它有利于蛋白质和细胞介导的治疗，并在体内刺激舌肌再生。并且在凝胶植入的动物中新再生的肌纤维显着增加。开发的生物材料平台显示出作为舌切除后再生治疗的巨大潜力，因为它有利于蛋白质和细胞介导的治疗，并在体内刺激舌肌再生。