Soybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo‐inositol 1‐phosphate synthase (Gm‐lpa‐TW75‐1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm‐lpa‐TW75‐1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm‐lpa‐TW75‐1. Phosphate (Pi) is higher in Gm‐lpa‐TW75‐1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm‐lpa‐TW75‐1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.

中文翻译：

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具有斑鸠菊二酰基甘油酰基转移酶-1 基因和肌醇 1-磷酸合酶敲除突变的大豆品系的产生和表征。

大豆 ( Glycine max ) 粉是重要的蛋白质来源。具有较低植酸盐和低聚糖的豆粕可提高豆粕质量。在大豆中的单个隐性突变肌肉肌醇-1-磷酸合酶（跨导LPA -TW75-1）赋予具有低肌醇六磷酸酯种子表型和增加的无机磷酸盐。该突变体与表达来自斑鸠菊属植物(VgD)的二酰基甘油酰基转移酶 1 (DGAT) 基因的高油品系杂交。转基因‐TW75‐1 X VgD，指定为 GV，分别有 21% 和 22% 的油以及 41% 和 43% 的蛋白质来自田间和温室种子生产。除了 Fe 之外，其他矿物浓度没有显着差异，GV 为 229 μg/g 干重，VgD 为 174.3，Gm-lpa- TW75-1为 162 。磷酸盐 (Pi) 在Gm-lpa- TW75-1 中较高，为 5 mg/g，其次是 GV，为 1.6 mg/g，而 Jack、VgD 和 Taiwan75 约为 0.3 mg/g。所述跨导LPA -TW75-1线具有在1.4的最低浓度植酸毫克克，随后用GV 1.8 /毫克/克，比Taiwan75，VGD和杰克用2.5毫克/克。这项工作描述了一种高油和蛋白质大豆品系 GV，它具有增加的 Pi 和较低的植酸盐，这将增加人类和动物饲料的营养价值。