Gene transcription in bacteria is mainly triggered by sigma factors (σ factors), such as rpoE. Bacterial ncRNAs are key players in reprogramming protein transcription when the environment changes. In our experiment, under the stress of ampicillin, the ncRNA transcriptomes of Salmonella enterica serovar Typhi wild-type strain (WT) and rpoE-deficient strain (ΔrpoE) were sequenced and analyzed, and four ncRNAs were selected to be verified by qRT-PCR. Of the ncRNA transcripts tested, 57 ncRNAs were found to have significantly different expressions (fold changes > 2) in ΔrpoE compared to WT, with 31 being upregulated and 26 being downregulated. The expression levels of the four ncRNAs verified preliminarily by subsequent qRT-PCR showed consistency with the sequencing data. Our study revealed the differences in ncRNA expression profiles between Salmonella enterica serovar Typhi WT and ΔrpoE under ampicillin stress. The four ncRNAs identified by qRT-PCR and their associated signaling pathways may be related to the envelope stress and antibiotic susceptibility of Salmonella enterica serovar Typhi.

中文翻译：

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氨苄青霉素胁迫下rpoE对伤寒沙门氏菌非编码RNA表达谱的影响

细菌中的基因转录主要由 sigma 因子（σ 因子）触发，例如 rpoE。当环境变化时，细菌 ncRNA 是重新编程蛋白质转录的关键参与者。在我们的实验中，在氨苄青霉素的胁迫下，对伤寒沙门氏菌血清型野生型菌株（WT）和rpoE缺陷型菌株（ΔrpoE）的ncRNA转录组进行了测序和分析，并选择了四种ncRNA进行qRT-PCR验证. 在测试的 ncRNA 转录本中，发现 57 种 ncRNA 在 ΔrpoE 中与 WT 相比具有显着不同的表达（倍数变化 > 2），其中 31 种被上调，26 种被下调。经后续qRT-PCR初步验证的4种ncRNA的表达水平与测序数据一致。我们的研究揭示了在氨苄青霉素胁迫下，伤寒沙门氏菌血清型 WT 和 ΔrpoE 之间 ncRNA 表达谱的差异。qRT-PCR鉴定的四种ncRNA及其相关信号通路可能与伤寒沙门氏菌的包膜应激和抗生素敏感性有关。