A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns. The user identifies a region of interest (ROI) by drawing on a live video image of the sample with the computer mouse. Motion commands are automatically generated to move the sample to acquire scan data for the pixels in the ROI. Synchronization of sample stage motion with laser firing and mass spectrometer (MS) scan acquisition is fully automated. RastirX saves a co-registered optical image and the scan location information needed to convert raw MS data into imzML format. Imaging an arbitrarily shaped ROI instead of the minimal enclosing rectangle reduces contamination from off-sample material and significantly reduces acquisition time.

中文翻译：

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用于任意空间模式质谱成像的多功能平台。

已经构建了一个视觉系统驱动平台 RastirX，用于任意二维模式的质谱成像 (MSI)。用户通过使用计算机鼠标在样本的实时视频图像上绘制来识别感兴趣区域 (ROI)。自动生成运动命令以移动样本以获取 ROI 中像素的扫描数据。样品台运动与激光发射和质谱仪 (MS) 扫描采集的同步是全自动的。RastirX 保存共同注册的光学图像和将原始 MS 数据转换为 imzML 格式所需的扫描位置信息。对任意形状的 ROI 而不是最小封闭矩形进行成像可以减少样品外材料的污染，并显着缩短采集时间。