Background: For the past several decades, the presence of tuberculosis (TB) is being remarked as the most common infectious disease leading to mortality.

Objective: Hydrazone containing azometine group (-NHN=CH-) compounds have been reported for a broad range of bioactivities such as antiplatelet, analgesic, anti-inflammatory, anticonvulsant, antidepressant, antimalarial, vasodilator, antiviral, and antimicrobial, etc.

Methods: For the synthesis of compounds (4a-4d) and (6a-6e), aromatic amines were treated with methyl terephthalaldehydate in methanol, giving Schiff’s bases, followed by reductive amination and further treatment with hydrazine hydrate gave acid hydrazides (4a-4d). These acid hydrazides were then treated with different aromatic aldehydes to yield hydrazones (6a-6d). All the synthesized compounds were subjected to FT-IR, NMR, and UV spectroscopic characterization.

Results: Compounds (4a-4d) and (6a-6e) were found to have highly potent activity against Mycobacteria tuberculosis (Vaccine strain, H37 RV strains): ATCC No- 27294 (MIC:1.6-6.25 μg/mL) than standard anti-TB drugs. The compounds exhibited good radical scavenging potentials(0- 69.2%), as checked from DPPH protocol. All compounds also demonstrated good in-silico ADMET results.

Conclusion: The current study revealed promising in vitro anti-tuberculosis and anti-oxidant profiles of hydrazide-hydrazone analogues.

背景：在过去的几十年中，结核病 (TB) 的存在被认为是导致死亡的最常见传染病。

目的：含氮唑汀基（-NHN=CH-）的腙类化合物具有广泛的生物活性，如抗血小板、镇痛、抗炎、抗惊厥、抗抑郁、抗疟疾、血管扩张剂、抗病毒和抗菌等。

方法：为了合成化合物（4a-4d）和（6a-6e），芳香胺在甲醇中用对苯二甲酸甲酯处理，得到席夫碱，然后还原胺化，再用水合肼处理得到酰肼（4a-4d） ）。然后用不同的芳香醛处理这些酸酰肼以产生腙（6a-6d）。所有合成的化合物都进行了 FT-IR、NMR 和 UV 光谱表征。

结果：发现化合物（4a-4d）和（6a-6e）对结核分枝杆菌（疫苗株，H37 RV株）具有高效活性：ATCC No-27294（MIC：1.6-6.25 μg/mL）比标准抗- 结核病药物。从 DPPH 协议检查，化合物表现出良好的自由基清除潜力 (0-69.2%)。所有化合物也表现出良好的计算机 ADMET 结果。

结论：目前的研究揭示了酰肼-腙类似物的有希望的体外抗结核和抗氧化特性。