Aims and Objectives: The intake of Stachys sieboldii MIQ. has been associated with relieving inflammation and maintaining optimal gut health function. We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice. In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.

Materials and Methods: 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base. The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit. Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.

Results: Mice fed on S. sieboldii MIQ. showed significantly reduced counts of aerobic and coliform in the feces compared with control. 16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ. increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.) in feces compared with that in the control (P<0.05 for all). Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05). The fecal fatty acid composition in the S. sieboldii MIQ. group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05). The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ. group (P<0.05). No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.

Conclusion: The present results showed that supplementation of mice with S. sieboldii MIQ. increased beneficial gut microflora and decreased harmful microflora. Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.

目的和目标：Stachys sieboldii MIQ 的摄入量。与缓解炎症和维持最佳肠道健康功能有关。我们调查了 S. sieboldii MIQ. 喂养小鼠粪便中微生物群落的多样性和组成。此外，我们评估了与炎症和几种组织的脂肪酸组成相关的主要细胞因子（白细胞介素 6 和 -10）的产生。

材料和方法：使用 EzBioCloud 数据库进行基于 16S 核糖体 DNA 测序的微生物组分类学分析。从肠系膜淋巴结中分离出总RNA，然后用prime script 第一链cDNA合成试剂盒合成。使用 SYBR™ Green PCR Master Mix 对 cDNA 样品进行定量实时 PCR。

结果：小鼠以 S. sieboldii MIQ 为食。与对照组相比，粪便中需氧菌和大肠菌群的数量显着减少。粪便样本的 16S rDNA 测序分析表明，补充了 S. sieboldii MIQ。与对照组相比，粪便中有益肠道菌群（Ruminococcaceae 和 Akkermansia muciniphila）增加并减少了有害菌群（肠杆菌科，包括大肠杆菌和拟杆菌属）（P<0.05）。与对照组相比，小鼠肠系膜淋巴结中细胞因子IL-6和IL-10的mRNA表达显着降低（P<0.05）。S. sieboldii MIQ 中的粪便脂肪酸组成。6:0 和 18:2n-6 的比例高于对照组（P<0.05）。6:0 和 20 的百分比：3n-6 脂肪酸在 S. sieboldii MIQ 的肠道中也显着更高。组（P<0.05）。在粪便和组织中发现的总饱和、单不饱和、n-6 和 n-3 多不饱和脂肪酸的百分比方面，两组之间没有显示差异。

结论：目前的结果表明小鼠补充了 S. sieboldii MIQ。增加有益肠道菌群，减少有害菌群。此外，补充小鼠肠系膜淋巴结中促炎细胞因子 IL-6 和抗炎细胞因子 IL-10 的 mRNA 表达较低可能与有害粪便微生物群落的丰度较低有关。