Abstract

Biomedical devices trigger immune responses when implanted in the body, as they are treated as foreign bodies. To avoid inflammatory responses and enhance the biocompatibility of biomedical devices, advanced coating technology that can modulate immune responses is essential. As a part of the immune response in the body, autologous cells evade attack from macrophages using CD47 ligands that function as markers for self. Inspired by this self-recognition system, we developed a camouflage coating for biomaterial surfaces using cell membrane vesicles that could suppress inflammatory responses. In this study, we used monocyte-derived cell membrane vesicles expressing CD47 for coating nanocellulose-coated substrates. Our data showed that presentation of CD47 to macrophages elicited negative signal transduction for immunosuppression. Further, for coating, we used cell membrane vesicles and plant-derived nanofibers. We observed that the supporting layer of cellulose nanofibers physically fixed cell membrane vesicles and provided hydrophilic surfaces to the polystyrene substrate. Based on CD47 signaling, cell membrane vesicle coating suppressed the inflammatory responses of stimulated macrophages. Camouflaging biomaterial surfaces with cell-derived components might serve as an advanced coating platform to suppress inflammatory responses and enhance tissue integrity for biomedical devices after implantation.

摘要

生物医学设备在植入体内时会触发免疫反应，因为它们被视为异物。为了避免炎症反应并增强生物医疗器械的生物相容性，可以调节免疫反应的先进涂层技术必不可少。作为体内免疫反应的一部分，自体细胞使用作为自身标记物的 CD47 配体逃避巨噬细胞的攻击。受这种自我识别系统的启发，我们使用可以抑制炎症反应的细胞膜囊泡为生物材料表面开发了一种伪装涂层。在这项研究中，我们使用单核细胞衍生的细胞膜囊泡表达 CD47 来涂覆纳米纤维素涂层的基材。我们的数据表明，CD47 向巨噬细胞的呈递引发了免疫抑制的负信号转导。此外，对于涂层，我们使用了细胞膜囊泡和植物来源的纳米纤维。我们观察到纤维素纳米纤维的支撑层物理固定细胞膜囊泡，并为聚苯乙烯基材提供亲水表面。基于 CD47 信号，细胞膜囊泡涂层抑制了受刺激的巨噬细胞的炎症反应。用细胞衍生成分伪装生物材料表面可能作为一种先进的涂层平台，以抑制炎症反应并增强植入后生物医学设备的组织完整性。细胞膜囊泡涂层抑制刺激巨噬细胞的炎症反应。用细胞衍生成分伪装生物材料表面可能作为一种先进的涂层平台，以抑制炎症反应并增强植入后生物医学设备的组织完整性。细胞膜囊泡涂层抑制刺激巨噬细胞的炎症反应。用细胞衍生成分伪装生物材料表面可能作为一种先进的涂层平台，以抑制炎症反应并增强植入后生物医学设备的组织完整性。