Contamination with polycyclic aromatic hydrocarbons (PAHs) causes noticeable ecological problems in aquatic ecosystems. 9,10-Phenanthrenequione (9,10-PQ) is an oxidized PAH and is highly toxic to aquatic animals. However, the effects of 9,10-PQ on the molecular metabolism of fish remain largely unknown. In this study, Takifugu obscurus juveniles were acutely exposed to 44.30 µg/L 9,10-PQ for 3 days. The transcriptome profile changes in their livers were compared between the 9,10-PQ treatment group and the control using T. rubripes as the reference genome. The results identified 22,414 genes in our transcriptome. Among them, 767 genes were differentially expressed after exposure to 9,10-PQ, which enriched 16 KEGG pathways. Among them, the glycolysis, phagosome, and FOXO signaling pathways were significantly activated in 9,10-PQ treatment compared with the control. These data indicate that 9,10-PQ increased the glycolysis capacity to produce more energy for resistance and harmed immune function. Moreover, several genes related to tumorigenesis were significantly upregulated in response to 9,10-PQ, displaying the carcinogenic toxicity of 9,10-PQ to T. obscurus. Genes in steroid biosynthesis pathways were downregulated in the 9,10-PQ treatment group, suggesting interference with the endocrine system. Overall, these findings provide information to help evaluate the environmental risks that oxygenated-PAHs present to T. obscurus.

中文翻译：

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急性暴露于氧化的PAH 9,10-菲基喹酮后，暗淡aki草肝脏的转录组变化。

多环芳烃（PAHs）的污染在水生生态系统中引起了明显的生态问题。9,10-菲醌（9,10-PQ）是一种氧化的PAH，对水生动物有剧毒。但是，9,10-PQ对鱼类分子代谢的影响仍然未知。在这项研究中，暗色T鱼（Takifugu obscurus）幼虫在44.30 µg / L 9,10-PQ下急性暴露3天。比较了9,10-PQ治疗组和使用丁香橡胶的对照组肝脏的转录组谱变化。作为参考基因组。结果在我们的转录组中鉴定出22,414个基因。其中，暴露于9,10-PQ后，差异表达了767个基因，丰富了16条KEGG通路。其中，与对照组相比，在9,10-PQ处理中糖酵解，吞噬体和FOXO信号通路被显着激活。这些数据表明9,10-PQ增加了糖酵解能力，以产生更多的能量用于抵抗和损害免疫功能。此外，响应于9,10-PQ，一些与肿瘤发生有关的基因被显着上调，显示出9,10-PQ对T. obscurus的致癌毒性。。9,10-PQ治疗组中类固醇生物合成途径中的基因被下调，表明干扰内分泌系统。总体而言，这些发现提供了有助于评估含氧多环芳烃对隐孢子虫的环境风险的信息。