Salmonella developed drug-resistance under durative antibiotic pressures pressure. The widespread prevalence of Salmonella has been associated with not only drug-resistance but also pathogenicity. Outer membrane porin proteins (OMPs) are critical for the drug resistance of bacteria. Virulence genes in Salmonella pathogenicity islands (SPIs) play key roles in the virulence of bacteria. In this study, we analyzed the expression levels of three critical genes in ciprofloxacin-resistant strains and ciprofloxacin-susceptible strains of Salmonella, including outer membrane porin protein F (ompF), virulence genes invA and invE. In the clinical ciprofloxacin-resistant strains of Salmonella, the expression level of ompF was decreased. Meanwhile, the expression levels of invA and invE were decreased except for only one strain, indicating generally decreased virulence. These results were also verified with ciprofloxacin-induced resistant strains. Thus, it was informative for understanding the drug-resistance in Salmonella. Monitoring drug-resistance and virulence relevant genes would be significant in the prevention and control of salmonellosis.

中文翻译：

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ompF、invA和invE的mRNA表达与沙门氏菌对环丙沙星的耐药性相关

沙门氏菌在持续的抗生素压力下产生耐药性。沙门氏菌的广泛流行不仅与耐药性有关，而且与致病性有关。外膜孔蛋白 (OMP) 对细菌的耐药性至关重要。沙门氏菌致病岛 (SPI) 中的毒力基因在细菌毒力中起关键作用。在这项研究中，我们分析了沙门氏菌环丙沙星耐药菌株和环丙沙星敏感菌株中三个关键基因的表达水平，包括外膜孔蛋白 F（ompF）、毒力基因 invA 和 invE。在临床耐环丙沙星沙门氏菌菌株中，ompF的表达水平降低。同时，除了只有一种菌株外，invA 和 invE 的表达水平下降，表明毒力普遍降低。这些结果也用环丙沙星诱导的耐药菌株进行了验证。因此，它有助于了解沙门氏菌的耐药性。监测耐药性和毒力相关基因对于沙门氏菌病的预防和控制具有重要意义。