In this work a simple and inexpensive method to assess the concentration ratio of the labile and mineral-bound microelements of the bone tissue was developed. The approach is based on the separation of the components of bone tissue by their selective solubility with the subsequent determination of microelements with atomic absorption spectrometry. The total concentrations of Mg, Zn, Fe, Sr, Al, Cu, and Mn and the concentrations of these elements in aqueous solutions with pH 6.5, 10, and 12 after their ultrasonically activated interaction with the powder of dried bone were determined. Two quite different bone samples were analyzed: a cortical fragment of the femur of a mature healthy cow and the spongy part of a human femoral head affected by osteoporosis. Some common and individual features of the both type of bones in regard to the total concentrations and fractional distribution of microelements are discussed. The obtained concentrations of the "soluble" fractions of microelements were critically analyzed taking into account the possible reactions leading to new insoluble phases' formation in alkaline solutions. Based on the data obtained, the ability of elements to form labile fractions in the bone tissue could be arranged in the following descending series: Mg ≥ Zn > Al > Fe > Mn > Cu > Sr.

中文翻译：

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通过原子吸收光谱法测定骨组织中不稳定和矿物质结合微量元素分数的简单方法。

在这项工作中，开发了一种简单且廉价的方法来评估骨组织中不稳定和矿物质结合的微量元素的浓度比。该方法基于通过其选择性溶解度分离骨组织成分，然后用原子吸收光谱法测定微量元素。测定了 Mg、Zn、Fe、Sr、Al、Cu 和 Mn 的总浓度以及这些元素在 pH 为 6.5、10 和 12 的水溶液中与干骨粉末发生超声活化相互作用后的浓度。分析了两个完全不同的骨骼样本：成熟健康牛股骨的皮质碎片和受骨质疏松症影响的人类股骨头的海绵部分。讨论了这两种骨骼在微量元素的总浓度和分数分布方面的一些共同和个别特征。考虑到可能导致在碱性溶液中形成新的不溶相的反应，对微量元素的“可溶”部分的获得浓度进行了严格分析。根据获得的数据，元素在骨组织中形成不稳定部分的能力可以按以下降序排列：Mg ≥ Zn > Al > Fe > Mn > Cu > Sr。在碱性溶液中形成。根据获得的数据，元素在骨组织中形成不稳定部分的能力可以按以下降序排列：Mg ≥ Zn > Al > Fe > Mn > Cu > Sr。在碱性溶液中形成。根据获得的数据，元素在骨组织中形成不稳定部分的能力可以按以下降序排列：Mg ≥ Zn > Al > Fe > Mn > Cu > Sr。