In Argentina, many Flavivirus were recognised including West Nile virus (WNV). During 2009 several strains of Culex Flavivirus (CxFV), an insect-specific flavivirus, were isolated in the same region where circulation of WNV was detected. Hence, the objective of this study was to analyse the effect of co-infection in vitro assays using CxFV and WNV Argentinean strains in order to evaluate if CxFV could affect WNV replication. Our results showed that WNV replication was suppressed when multiplicity of infection (MOI) for CxFV was 10 or 100 times higher than WNV. Nevertheless, in vivo assays are necessary in order to evaluate the superinfection exclusion potential.

中文翻译：

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使用来自阿根廷的西尼罗河病毒和库克斯黄病毒株进行的超级感染排除研究。

在阿根廷，人们认识到许多黄病毒，包括西尼罗河病毒（WNV）。在2009年期间，在检测到WNV传播的同一区域中分离出了几株昆虫特异性黄病毒库克斯黄病毒（CxFV）。因此，本研究的目的是分析使用CxFV和WNV阿根廷菌株的体外共感染的效果，以评估CxFV是否会影响WNV复制。我们的结果表明，当CxFV的感染复数（MOI）比WNV高10或100倍时，WNV复制受到抑制。但是，为了评估重叠感染的可能性，必须进行体内分析。