Here, we describe a novel microfilter device to capture circulating tumor cells in an efficient and low-cost manner. Then, we validated the safety and clinical utility of the novel microfilter device. We next performed mutation analysis from circulating tumor cells collected from lung cancer patients using this new device. Our results indicate that this microfilter system can be used to investigate the genome landscape of circulating tumor cells collected from lung cancer patients. Further, our results highlight a proof-of-concept demonstration indicating that circulating tumor cell can be used for mutation profiling during tumor evolution, therapy prediction, and monitoring, with immediate clinical applicability.

在这里，我们描述了一种新型微过滤器装置，以高效且低成本的方式捕获循环肿瘤细胞。然后，我们验证了新型微过滤器装置的安全性和临床实用性。接下来，我们使用这种新设备对从肺癌患者身上收集的循环肿瘤细胞进行了突变分析。我们的结果表明，该微过滤系统可用于研究从肺癌患者收集的循环肿瘤细胞的基因组图谱。此外，我们的结果强调了一个概念验证证明，表明循环肿瘤细胞可用于肿瘤进化、治疗预测和监测过程中的突变分析，具有直接的临床适用性。