Reptiles show a remarkable diversity of sex determination mechanisms and sex chromosome systems, derived from different autosomal pairs. The origin of the ZW sex chromosomes of Lacerta agilis, a widespread Eurasian lizard species, is a matter of discussion: is it a small macrochromosome from the 11-18 group common to all lacertids, or does this species have a unique ZW pair derived from the large chromosome 5? Using independent molecular cytogenetic methods, we investigated the karyotype of L. agilis exigua from Siberia, Russia, to identify the sex chromosomes. FISH with a flow-sorted chromosome painting probe derived from L. strigata and specific to chromosomes 13, 14, and Z confirmed that the Z chromosome of L. agilis is a small macrochromosome, the same as in L. strigata. FISH with the telomeric probe showed an extensive accumulation of the telomere-like repeat in the W chromosome in agreement with previous studies, excluding the possibility that the lineages of L. agilis studied in different works could have different sex chromosome systems due to a putative intra-species polymorphism. Our results reinforce the idea of the stability of the sex chromosomes and lack of evidence for sex-chromosome turnovers in known species of Lacertidae.

中文翻译：

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染色体绘画不支持Lacerta agilis Linnaeus的性染色体周转，1758 年

爬行动物显示出来自不同常染色体对的性别决定机制和性染色体系统的显着多样性。Lacerta agilis 是一种广泛存在的欧亚蜥蜴物种，其 ZW 性染色体的起源是一个讨论的问题：它是来自所有蝎虎科动物共有的 11-18 组的小大染色体，还是该物种有一个独特的 ZW 对源自大染色体5？我们使用独立的分子细胞遗传学方法研究了来自俄罗斯西伯利亚的 L. agilis exigua 的核型，以确定性染色体。FISH 用流式分选的染色体涂漆探针来自 L. strigata 并特异于 13、14 和 Z 染色体，证实 L. agilis 的 Z 染色体是一个小的大染色体，与 L. strigata 相同。使用端粒探针的 FISH 显示 W 染色体中端粒样重复序列的广泛积累与先前的研究一致，排除了在不同工作中研究的 L. agilis 谱系可能具有不同性染色体系统的可能性。 -物种多态性。我们的结果强化了性染色体稳定性的观点，并且缺乏已知蜘蛛科物种中性染色体更替的证据。