Autologous nerve grafting is the golden standard therapeutic approach of peripheral nerve injury. However, the clinical effect of autologous nerve grafting is still unsatisfying. To achieve better clinical functional recovery, it is of an impending need to expand our understanding of the dynamic cellular and molecular changes after nerve transection and autologous nerve transplantation. To address this aim, in the current study, rats were subjected to sciatic nerve transection and autologous nerve grafting. Rat sciatic nerve segments were collected at 4, 7, and 14 days after surgery and subjected to antibody array analysis to determine phosphoprotein profiling patterns. Compared with rats that underwent sham surgery, a total of 48, 19, and 75 differentially expressed phosphoproteins with fold changes > 2 or < −2 were identified at 4, 7, and 14 days after autologous nerve grafting, respectively. Several phosphoproteins, including STAM2 (Phospho-Tyr192) and Tau (Phospho-Ser422), were found to be differentially expressed at multiple time points, suggesting the importance of the phosphorylation of these proteins. Western blot validation of the expression patterns of STAM2 (Phospho-Tyr192) indicated the accuracy of antibody array assay. Bioinformatic analysis of these differentially expressed proteins suggested that cellular behavior and organ morphology were significantly involved biological functions while cell behavior and immune response-related signaling pathways were significantly involved canonical signaling pathways. These outcomes contributed to the illumination of the molecular mechanisms underlying autologous nerve grafting from the phosphoprotein profiling perspective.

自体神经移植是治疗周围神经损伤的金标准方法。然而自体神经移植的临床效果仍不理想。为了实现更好的临床功能恢复，迫切需要扩大我们对神经横断和自体神经移植后动态细胞和分子变化的理解。为了实现这一目标，在当前的研究中，对大鼠进行了坐骨神经横断和自体神经移植。手术后 4、7 和 14 天收集大鼠坐骨神经节段，并进行抗体阵列分析以确定磷蛋白分析模式。与接受假手术的大鼠相比，自体神经移植后第4、7和14天分别鉴定出48、19和75个差异表达磷蛋白，其倍数变化> 2或< -2。多种磷蛋白，包括 STAM2 (Phospho-Tyr192) 和 Tau (Phospho-Ser422)，被发现在多个时间点有差异表达，表明这些蛋白磷酸化的重要性。 STAM2 (Phospho-Tyr192) 表达模式的蛋白质印迹验证表明了抗体阵列测定的准确性。对这些差异表达蛋白的生物信息分析表明，细胞行为和器官形态显着参与生物学功能，而细胞行为和免疫反应相关信号通路显着参与经典信号通路。这些结果有助于从磷蛋白分析的角度阐明自体神经移植的分子机制。