Protein adsorption to biomaterial surfaces is important for the function of such materials with anchorage-dependent cell adhesion requiring the presence of adsorbed proteins. The current study evaluated five solid surfaces with poly(acrylic acid) (PAA) grafted from the surface of a poly(tetrafluoroethylene) membrane with respect to the adsorption of serum albumin (SA), lactoferrin (Lf), and lysozyme (Lys) from a phosphate buffer and NaCl solution or water for specific combinations. With the use of x-ray photoelectron spectroscopy, the relative amounts and protein layer thickness were evaluated. SA adsorption was governed by ionic repulsive forces and hydrophobic interactions as evidenced from an increase in the protein adsorption at lower pH (6.5 compared to 7.4) and a correlation with surface coverage when water (pH 6.5) was used as the medium. The adsorption of Lf and Lys followed similar trends for all samples. In general, ionic attractive forces dominated and a strong correlation of increasing protein adsorption with the PAA chain length was evident. This study concluded that all surfaces appear suitable for use in biomaterial applications where tissue ingrowth is desired and that the enhanced protein adsorption in a medium with high ionic strength (e.g., biological fluid) correlates with the PAA chain length rather than the surface coverage.

中文翻译：

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蛋白质吸附到接枝了聚丙烯酸链的聚四氟乙烯膜上。

蛋白质吸附到生物材料表面对于这种材料的功能很重要，这种材料具有依赖于锚定的细胞粘附，需要存在吸附的蛋白质。本研究评估了从聚四氟乙烯膜表面接枝的聚丙烯酸（PAA）在五个固体表面上的吸附血清白蛋白（SA），乳铁蛋白（Lf）和溶菌酶（Lys）的能力。磷酸盐缓冲液和氯化钠溶液或水的特定组合。使用X射线光电子能谱，评估了相对量和蛋白质层厚度。SA吸附受离子排斥力和疏水相互作用的控制，从较低p处蛋白质吸附的增加可以证明H（6.5比7.4）和以水（p H 6.5）作为介质时表面覆盖率的相关性。对于所有样品，Lf和Lys的吸附遵循相似的趋势。通常，离子吸引力占主导，并且明显的蛋白质吸附增加与PAA链长密切相关。这项研究得出的结论是，所有表面似乎都适合用于需要组织向内生长的生物材料应用，并且在具有高离子强度的介质（例如，生物流体）中增强的蛋白质吸附与PAA链长而不是表面覆盖率相关。