Background: Bacteriocins (Bac1, Bac2, and Bac3) from Weissella confusa MBF8-1, weissellicin- MBF, have been reported as potential alternative substances as well as complements to the existing antibiotics against many antimicrobial-resistant pathogens. Previously, the genes encoded in the large plasmid, pWcMBF8-1, and the spermicidal activity of their synthetic peptides, originally discovered Indonesia, have been studied. Three synthetic bacteriocins peptides of this weissellicin-MBF have been reported for their potential activities, i.e. antibacterial and spermicidal.

Objective: The aim of this study was to construct the recombinant Bacteriocin (r-Bac) genes, as well as to investigate the gene expressions and their functional analysis.

中文翻译：

---

魏氏菌MBF8-1重组细菌魏霉素MBF的构建及功能分析

背景：Weissella confusa MBF8-1，weissellicin-MBF的细菌素（Bac1，Bac2和Bac3）已被报道为潜在的替代物质，并且是针对许多抗药性病原体的现有抗生素的补充。以前，已经研究了大质粒pWcMBF8-1中编码的基因及其最初发现于印度尼西亚的合成肽的杀精活性。已经报道了该魏斯霉素-MBF的三种合成细菌素肽的潜在活性，即抗菌和杀精子。

目的：本研究的目的是构建重组细菌素（r-Bac）基因，并研究其基因表达及其功能分析。