Stem cell-based regenerative medicine is a promising approach for tissue reconstruction. However, a large number of cells are needed in a typical clinical study, where conventional monolayer cultures might pose a limitation for scale-up. The purpose of this review was to systematically assess the application of microcarriers in Mesenchymal Stem Cell cultures. A comprehensive search was conducted in Medline via Ebscohost, Pubmed, and Scopus, and relevant studies published between 2015 and 2019 were selected. The literature search identified 53 related studies, but only 14 articles met the inclusion criteria. These include 7 utilised commercially available microcarriers, while the rest were formulated based on different surface characteristics, all of which are discussed in this review. Current applications of microcarriers were focused on MSC expansion and induction of MSCs into different lineages. These studies demonstrated that MSCs could proliferate in a microcarrier culture system in-fold compared to monolayer cultures, and the culture system could simulate a three-dimensional environment which induces cell differentiation. However, detailed studies are still required before this system were to be adapted into the scale of GMP manufacturing.

中文翻译：

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间充质干细胞培养中的三维微载体系统：系统综述。

基于干细胞的再生医学是用于组织重建的有前途的方法。但是，在典型的临床研究中，需要大量细胞，而常规的单层培养可能会限制放大规模。这篇综述的目的是系统地评估微载体在间充质干细胞培养中的应用。在Medline上通过Ebscohost，Pubmed和Scopus进行了全面搜索，并选择了2015年至2019年之间发表的相关研究。文献检索确定了53项相关研究，但只有14篇文章符合纳入标准。这些包括7种可利用的市售微载体，其余的则根据不同的表面特性进行配制，所有这些均在本综述中进行了讨论。微载体的当前应用集中在MSC扩增和将MSC诱导成不同谱系中。这些研究表明，与单层培养相比，MSC可以在微载体培养系统中增殖，并且培养系统可以模拟诱导细胞分化的三维环境。但是，在将该系统应用于GMP制造规模之前，仍然需要进行详细的研究。