BACKGROUND CD8+ regulatory T cells (CD8+ Tregs) are relatively recently described T cell subsets that have been shown to regulate various T cell responses and appear to play a role in autoimmunity. However, their effects on B cells have not been explored. OBJECTIVES In this investigation we examine the effect of CD8+ Tregs on various subsets of peripheral B cells include naïve B cells, transitional B cells, marginal zone B cells, IgM memory B cells, class switched memory B cells, and plasmablasts, and on the expression of B cell-activating factor receptor (BAFF-R). METHODS CD8+ T cells were first purified and then activated with anti-CD3/CD28 beads to generate CD8+ Tregs. Purified CD19+ B cells were cultured alone or with sorted CD8+ Tregs (CD8+CD183+CCR7+CD45RA-) and activated with anti-CD40 monoclonal antibody and CpG. B cell subsets and the expression of BAFF-R on naïve and memory B cells were analyzed using various monoclonal antibodies and corresponding control isotypes. Ten thousand cells were acquired and analyzed by FACSCalibur using the FlowJo software. RESULTS CD8+ Tregs selectively and significantly suppressed plasmablasts without any significant effect on other B cell subsets or on the expression of BAFF-R. CONCLUSION CD8+ Tregs may play a role in autoimmunity by regulating antibody production via suppression of plasmablasts.

中文翻译：

---

CD8 +调节性T细胞对人B细胞亚群的体外作用。

背景技术CD8 +调节性T细胞（CD8 + Tregs）是最近才描述的T细胞亚群，已被证明可调节各种T细胞反应并似乎在自身免疫中发挥作用。然而，尚未研究它们对B细胞的作用。目的在这项研究中，我们研究了CD8 + Treg对外周B细胞各种亚型的影响，包括幼稚B细胞，过渡性B细胞，边缘区B细胞，IgM记忆B细胞，类开关记忆B细胞和浆母细胞，以及它们的表达B细胞活化因子受体（BAFF-R）的表达。方法首先纯化CD8 + T细胞，然后用抗CD3 / CD28珠激活以产生CD8 + Treg。纯化的CD19 + B细胞可单独培养或与分选的CD8 + Tregs（CD8 + CD183 + CCR7 + CD45RA-）培养，并用抗CD40单克隆抗体和CpG激活。使用各种单克隆抗体和相应的对照同种型分析B细胞亚群以及幼稚和记忆B细胞上BAFF-R的表达。采集一万个细胞，并使用FlowJo软件通过FACSCalibur分析。结果CD8 + Tregs选择性地显着抑制了成浆细胞，而对其他B细胞亚群或BAFF-R的表达没有任何显着影响。结论CD8 + Tregs可能通过抑制成浆细胞来调节抗体产生，从而在自身免疫中发挥作用。结果CD8 + Tregs选择性地显着抑制了成浆细胞，而对其他B细胞亚群或BAFF-R的表达没有任何显着影响。结论CD8 + Tregs可能通过抑制成浆细胞来调节抗体产生，从而在自身免疫中发挥作用。结果CD8 + Tregs选择性地显着抑制了成浆细胞，而对其他B细胞亚群或BAFF-R的表达没有任何显着影响。结论CD8 + Tregs可能通过抑制成浆细胞来调节抗体产生，从而在自身免疫中发挥作用。