The precondition of studying biological sample is to extract sample metabolites by the best pretreatment methods. There is already limited information about pretreatments of fermented feed metabolites. The study compared the extraction effects of different pulverisation methods used in the sample pretreatment process for the extraction of metabolites from cottonseed meal fermented by Lactobacillus acidophilus based on UPLC-Q-TOF-MS. The extraction effects of three pretreatments (non-pulverisation (WF), pulverisation (F), and high-speed homogenisation methods (YJ)) were compared with the numbers of metabolites and the normalised peak areas of the metabolites. The results showed that the number of metabolites extracted with three pulverisation methods were 1745, 1896, 2132 (ESI+ mode) and 1447, 1675, 2073 (ESI− mode), respectively. The number of variable importance plot (VIP) metabolites and the relative peak areas of metabolites showed that the trend was YJ > F > WF. The extraction effect of high-speed homogenisation method was the best way to extract metabolites from the fermented cottonseed meal. This study built a foundation work for the further research of the fermented feed metabolomics.

中文翻译：

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基于UPLC-Q-TOF-MS的不同样品粉碎方法对发酵棉粕中代谢物提取的影响

研究生物样品的前提是采用最佳的前处理方法提取样品代谢物。关于发酵饲料代谢物预处理的信息已经很有限。本研究基于 UPLC-Q-TOF-MS 比较了在样品预处理过程中使用不同粉碎方法从嗜酸乳杆菌发酵的棉籽粕中提取代谢物的提取效果。将三种预处理（非粉碎 (WF)、粉碎 (F) 和高速匀浆方法 (YJ)）的提取效果与代谢物的数量和代谢物的归一化峰面积进行比较。结果表明，三种粉碎方法提取的代谢物数量分别为1745、1896、2132（ESI+模式）和1447、1675、2073（ESI-模式）。可变重要性图（VIP）代谢物的数量和代谢物的相对峰面积表明趋势是YJ > F > WF。高速均质法的提取效果是提取发酵棉籽粕代谢物的最佳方法。本研究为发酵饲料代谢组学的进一步研究奠定了基础。