Radix astragali is a popular traditional herbal medicine that provides significant protection against tissue injury in various models of oxidative stress-related diseases. In this study, we aimed to investigate whether administration of Radix astragali prevented atrophy in both slow- and fast-twitch muscles following cast immobilization. Twenty-seven 12-week-old male F344 rats were divided into three experimental groups: control (CON), immobilized (IM), and immobilized with Radix astragali administration (IM+AR). Rats in the IM and IM+AR groups were subjected to immobilization of both lower extremities using casting-tape for 14 days. Rats in the IM+AR group were orally administered a decoction of Radix astragali daily for 21 days beginning 7 days before cast immobilization. As expected, rats in the IM group showed significant decreases (P < 0.05) in soleus and plantaris muscle-to-body weight ratios by 74.3% and 70.5%, respectively, compared with those in the CON group. Administration of Radix astragali significantly reversed (+35.5%) the weight reduction observed in soleus muscle, but not in the plantaris muscle, compared with that in the IM group. Furthermore, administration of Radix astragali inhibited MuRF1 mRNA expression only in the soleus muscle during cast immobilization. Our results demonstrated that administration of Radix astragali suppressed the immobilization-induced reductions in skeletal muscle mass and expression of MuRF1 mRNA in slow-twitch soleus muscles, but not in fast-twitch plantaris muscles.

中文翻译：

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黄芪，草药对固定化引起的慢肌和快肌萎缩的保护作用不同。

黄芪是一种流行的传统草药，在各种氧化应激相关疾病模型中均能显着保护组织免受伤害。在这项研究中，我们旨在调查黄芪对石膏固定后是否能预防慢肌和快肌的萎缩。将28只12周大的雄性F344大鼠分为三个实验组：对照组（CON），固定化（IM）和黄芪给药（IM + AR）。IM和IM + AR组中的大鼠使用铸型带固定两个下肢14天。IM + AR组的大鼠在固定石膏前7天开始，每天口服黄芪汤21天。如预期的那样，IM组的大鼠表现出明显的下降（P <0。05）比目鱼组和plant肌的体重比分别比CON组高74.3％和70.5％。与IM组相比，黄芪注射液可显着逆转（+ 35.5％）在比目鱼肌中观察到的体重减轻，但在the肌中观察不到。此外，黄芪的施用仅在石膏固定期间抑制比目鱼肌中的MuRF1 mRNA表达。我们的结果表明，黄芪能抑制固定化诱导的慢肌比目鱼肌骨骼肌质量的减少和MuRF1 mRNA的表达，但不能抑制快肌动的比目鱼肌。与IM组相比，黄芪注射液可显着逆转（+ 35.5％）在比目鱼肌中观察到的体重减轻，但在the肌中观察不到。此外，黄芪的施用仅在石膏固定期间抑制比目鱼肌中的MuRF1 mRNA表达。我们的结果表明，黄芪能抑制固定化诱导的慢肌比目鱼肌骨骼肌质量的减少和MuRF1 mRNA的表达，但不能抑制快肌动的比目鱼肌。与IM组相比，黄芪注射液可显着逆转（+ 35.5％）在比目鱼肌中观察到的体重减轻，但在the肌中观察不到。此外，黄芪的施用仅在石膏固定期间抑制比目鱼肌中的MuRF1 mRNA表达。我们的结果表明，黄芪能抑制固定化诱导的慢肌比目鱼肌骨骼肌质量的减少和MuRF1 mRNA的表达，但不能抑制快肌动的比目鱼肌。