Objective . In this study, we demonstrate practical applications of a novel 3-dimensional neural probe for simultaneous electrophysiological recordings from the surface of the brain as well as deep intra-cortical tissue. We used this 3D probe to investigate signal propagation mechanisms between neuronal cells and their responses to stimuli in a 3D fashion. Approach . This novel probe leverage 2D thin-film microfabrication technique to combine an epi -cortical (surface) and an intra -cortical (depth) microelectrode arrays ( Epi-Intra ), that unfold into an origami 3D-like probe during brain implantation. The flexible epi-cortical component conforms to the brain surface while the intra-cortical array is reinforced with stiffer durimide polymer layer for ease of tissue penetration. The microelectrodes are made of glassy carbon material that is biocompatible and has low electrochemical impedance that is important for high fidelity neuronal recordings. T...

中文翻译：

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具有玻璃碳微电极的Epi-Intra神经探针有助于阐明3D体积组织中的神经编码和刺激编码。

目标。在这项研究中，我们演示了新型3维神经探针在大脑表面以及深层皮质内组织同时进行电生理记录的实际应用。我们使用该3D探针以3D方式研究神经元细胞之间的信号传播机制及其对刺激的反应。方法。这种新颖的探针利用2D薄膜微细加工技术，将表皮层（表面）和皮层内（深度）微电极阵列（Epi-Intra）结合在一起，在大脑植入过程中，这些微电极阵列会像折纸3D一样展开。柔性表皮成分顺应大脑表面，而皮层内阵列则用较硬的杜蕾姆聚合物层加强以方便组织穿透。微电极由生物相容的玻璃碳材料制成，具有低电化学阻抗，这对高保真神经元记录很重要。T ...