Lipidomics is a rapidly growing field, fueled by developments in analytical instrumentation and bioinformatics. To date, most researchers and industries have employed their own lipidomics workflows without a consensus on best practices. Without a community‐wide consensus on best practices for the prevention of lipid degradation and transformations through sample collection and analysis, it is difficult to assess the quality of lipidomics data and hence trust results. Clinical studies often rely on samples being stored for weeks or months until they are analyzed, but inappropriate sampling techniques, storage temperatures, and analytical protocols can result in the degradation of complex lipids and the generation of oxidized or hydrolyzed metabolite artifacts. While best practices for lipid stability are sample dependent, it is generally recommended that strategies during sample preparation capable of quenching enzymatic activity and preventing oxidation should be considered. In addition, after sample preparation, lipid extracts should be stored in organic solvents with antioxidants at −20 °C or lower in an airtight container without exposure to light or oxygen. This will reduce or eliminate sublimation, and chemically and physically induced molecular transformations such as oxidation, enzymatic transformation, and photon/heat‐induced degradation. This review explores the available literature on lipid stability, with a particular focus on human health and/or clinical lipidomic applications. Specifically, this includes a description of known mechanisms of lipid degradation, strategies, and considerations for lipid storage, as well as current efforts for standardization and quality insurance of protocols.

中文翻译：

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回顾样品制备过程中提高脂质稳定性的努力和确保脂质测量报告准确性的标准化努​​力。

在分析仪器和生物信息学的发展推动下，脂质组学是一个快速发展的领域。迄今为止，大多数研究人员和行业都采用了自己的脂质组学工作流程，但没有就最佳实践达成共识。如果没有全社区就通过样本收集和分析预防脂质降解和转化的最佳实践达成共识，就很难评估脂质组学数据的质量，从而难以信任结果。临床研究通常依赖于将样品储存数周或数月直到进行分析，但不适当的取样技术、储存温度和分析方案会导致复杂脂质的降解和氧化或水解代谢产物的产生。虽然脂质稳定性的最佳实践取决于样品，但 通常建议在样品制备过程中考虑能够淬灭酶活性和防止氧化的策略。此外，样品制备后，脂质提取物应在-20°C或更低的密闭容器中储存在含有抗氧化剂的有机溶剂中，不要暴露在光线或氧气中。这将减少或消除升华以及化学和物理诱导的分子转化，例如氧化、酶转化和光子/热诱导的降解。本综述探讨了有关脂质稳定性的现有文献，特别关注人类健康和/或临床脂质组学应用。具体来说，这包括对已知的脂质降解机制、策略和脂质储存注意事项的描述，