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Evaluation of RIDASCREEN® and RIDA®QUICK Helicobacter kits for Helicobacter pylori detection in stools.
European Journal of Clinical Microbiology & Infectious Diseases ( IF 4.5 ) Pub Date : 2020-06-10 , DOI: 10.1007/s10096-020-03943-3
Lucie Bénéjat 1 , Alice Buissonnière 1 , Astrid Ducournau 1 , Francis Mégraud 1, 2 , Emilie Bessède 1, 2 , Philippe Lehours 1, 2
Affiliation  

The diagnosis of Helicobacter pylori infection can be made by using noninvasive tests. The detection of bacterial antigens in stool samples is a technique proposed by some suppliers. The objective of this study was to evaluate retrospectively the performances of the commercially available RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter (R-Biopharm) kits in detecting H. pylori antigens in stool samples. A collection of 132 stools was used in this study: 94 stools obtained from H. pylori-negative patients and 38 stools from H. pylori-positive patients. The performances (sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)) were evaluated for the RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter kits in comparison with real-time PCR results performed on gastric biopsies as well as culture. Discordant results, with respect to H. pylori status, were checked on the same day as the test by repeating the procedure. All of the readings concerning the RIDA®QUICK Helicobacter tests were concordant between 3 users, i.e., 94/94 negative tests and 34/38 positive tests. RIDASCREEN® Helicobacter tests were negative for all 94 H. pylori-negative samples and positive for 35/38 positive stools. Reading of the RIDA®QUICK Helicobacter tests was not a problem in routine practice. The RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter kits show good performances and can be included in the armamentarium of diagnostic tests for H. pylori infection.



中文翻译:

评估用于粪便中幽门螺杆菌的RIDASCREEN®和RIDA®QUICK幽门螺杆菌试剂盒。

幽门螺杆菌感染的诊断可以通过使用非侵入性测试来进行。粪便样品中细菌抗原的检测是一些供应商提出的技术。这项研究的目的是回顾性评估市售的RIDA®QUICK幽门螺杆菌和RIDASCREEN®幽门螺杆菌(R-Biopharm)试剂盒检测粪便样本中幽门螺杆菌抗原的性能。的132个粪便的集合在此研究中使用:从得到的粪便94幽门螺杆菌阴性患者和38个粪便从幽门螺旋杆菌阳性患者。评估了RIDA®QUICKHelicobacter和RIDASCREEN®Helicobacter试剂盒的性能(敏感性,特异性,阳性预测值(PPV),阴性预测值(NPV)),并与在胃活检和培养物中进行的实时PCR结果进行了比较。关于幽门螺杆菌状态的不一致结果,在测试的同一天通过重复该程序进行检查。与RIDA®QUICKHelicobacter测试有关的所有读数均在3个用户之间一致,即94/94阴性测试和34/38阳性测试。RIDASCREEN®幽门螺杆菌检测对所有94例幽门螺杆菌均呈阴性阴性样品,大便35/38阳性。常规操作中,读取RIDA®QUICK幽门螺杆菌检测并不是问题。RIDA®QUICK幽门螺杆菌和RIDASCREEN®幽门螺杆菌试剂盒表现出良好的性能,可包含在幽门螺杆菌感染诊断测试的设备中。

更新日期:2020-06-10
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