Peptide-Cross-Linked Protein-Imprinted Polymers: Easy Template Removal and Excellent Imprinting Effect,CCS Chemistry

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Peptide-Cross-Linked Protein-Imprinted Polymers: Easy Template Removal and Excellent Imprinting Effect
CCS Chemistry ( IF 9.4 ) Pub Date : 2019-11-04 , DOI: 10.31635/ccschem.019.20190030
Rong Xu ₁ , Jiafeng Tian ₁ , Ying Guan ₁ , Yongjun Zhang _{1,

2}

Affiliation

Molecular imprinting of proteins remains a huge challenge because of two major obstacles: difficulty in template removal and low imprinting efficiency. Herein, we propose a new strategy to simultaneously overcome these two challenges by creating molecularly imprinted polymers (MIPs) with nanoscale shape-memorable imprint cavities. These novel MIPs were developed by simply cross-linking the polymers with a peptide cross-linker instead of commonly used cross-linkers. Due to the unique pH-induced helix– coil transition of the peptide cross-linker, adjusting the pH from 5.5 to 7.4 leads to an expansion of the imprint cavities, thus facilitating template removal. Returning the pH back to 5.5 restores the original size and shape of the imprint cavities due to the precise refolding of peptide. A template protein can therefore be readily removed under mild conditions, while simultaneously achieving a significantly improved imprinting effect.

中文翻译：

肽交联的蛋白质印迹聚合物：易于去除模板并具有出色的印迹效果

由于两个主要障碍，蛋白质的分子印迹仍然是一个巨大的挑战：模板去除困难和印迹效率低。在这里，我们提出了一种新的策略，通过创建具有纳米级可记忆形状的压印腔的分子压印聚合物（MIP），来同时克服这两个挑战。这些新型MIP是通过简单地将聚合物与肽交联剂而不是常用的交联剂交联而开发的。由于独特的pH诱导的肽交联剂螺旋-螺旋转变，将pH从5.5调节至7.4会导致印迹腔扩大，从而有利于模板去除。由于肽的精确重折叠，使pH恢复到5.5可恢复压印腔的原始大小和形状。

更新日期：2020-06-24

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