A novel near-infrared fluorescent probe with an improved Stokes shift for specific detection of Hg2+ in mitochondria.,Organic & Biomolecular Chemistry

当前位置： X-MOL 学术 › Org. Biomol. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A novel near-infrared fluorescent probe with an improved Stokes shift for specific detection of Hg2+ in mitochondria.
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2020-06-23 , DOI: 10.1039/d0ob00507j
Jin Gong ₁ , Chang Liu ₂ , Xiaojie Jiao ₂ , Song He ₂ , Liancheng Zhao ₁ , Xianshun Zeng ₃

Affiliation

The mercury ion (Hg²⁺), one of the most notorious heavy metal ions, not only causes environmental pollution, but also endangers human health. There is evidence that Hg²⁺ tends to accumulate in the mitochondria and to induce apoptosis. However, mitochondria-targeted near-infrared (NIR) fluorescent probes with large Stokes shifts are still scarcely described for the specific detection of Hg²⁺. In this work, a novel near-infrared fluorescent probe JRQNS with a large Stokes shift (78 nm) was reported, and applied for sensitive and specific detection of Hg²⁺ in mitochondria by incorporating an additional amine group with fused rings to rhodamine dyes to enhance the electron donating ability of amine groups. As expected, the probe exhibited high selectivity and sensitivity to Hg²⁺ with a detection limit as low as 1.5 nM and fast response times (3 min), revealing that JRQNS could be used as a practical probe for quantitative detection of Hg²⁺ in real-time. Importantly, JRQNS can be used as an efficient organelle-targeting probe for imaging Hg²⁺ in the mitochondria of living cells, and thus detect Hg²⁺ in real-time there. The application of the probe for its selective localization in mitochondria along with the nanomolar level of limit of detection to Hg²⁺ ions provided a potential tool for studying the cytotoxic mechanisms of Hg²⁺.

中文翻译：

一种新型近红外荧光探针，具有改进的斯托克斯位移，用于特异性检测线粒体中的 Hg2+。

汞离子（Hg ²⁺）是最臭名昭著的重金属离子之一，不仅造成环境污染，而且危害人类健康。有证据表明 Hg ²⁺倾向于在线粒体中积累并诱导细胞凋亡。然而，具有大斯托克斯位移的线粒体靶向近红外 (NIR) 荧光探针仍然很少被描述用于 Hg ²⁺的特异性检测。在这项工作中，报道了一种具有大斯托克斯位移（78 nm）的新型近红外荧光探针JRQNS，并将其应用于 Hg ^{2+ 的}灵敏和特异检测通过在罗丹明染料中加入一个带有稠环的额外胺基团来增强胺基团的给电子能力。正如预期的那样，该探针对 Hg ²⁺表现出高选择性和灵敏度，检测限低至 1.5 nM，响应时间快（3 分钟），表明JRQNS可用作定量检测 Hg ²⁺的实用探针。即时的。重要的是，JRQNS可用作有效的细胞器靶向探针，用于对活细胞线粒体中的Hg ²⁺进行成像，从而检测 Hg ²⁺在那里实时。探针在线粒体中的选择性定位以及对 Hg ²⁺离子的纳摩尔级检测限的应用为研究 Hg ²⁺的细胞毒性机制提供了潜在的工具。

更新日期：2020-07-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11