Precipitation-based colorimetric multiplex immunoassay in hydrogel particles.,Lab on a Chip

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Precipitation-based colorimetric multiplex immunoassay in hydrogel particles.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-06-23 , DOI: 10.1039/d0lc00325e
Yoon Ho Roh ₁ , Hyun Jee Lee , Ju Yeon Kim , Hyeon Ung Kim , Sun Min Kim , Ki Wan Bong

Affiliation

Despite a growing demand for more accessible diagnostic technologies, current methods struggle to simultaneously detect multiple analytes with acceptable sensitivity and portability. Colorimetric assays have been widely used due to their simplicity of signal readout, but the lack of multiplexibility has been a perpetual constraint. Meanwhile, particle-based assays offer multiplex detection by assigning an identity code to each analyte, but they often require lab-based equipment unsuitable for portable diagnostics. Here, by merging the two approaches, this paper reports a colorimetric multiplex immunoassay based on hydrogel microparticles that achieves the best of both worlds. The low-cost portable multiplex assay demonstrates sensitivities as high as and dynamic ranges greater than the lab-based enzyme-linked immunosorbent assay (ELISA). These critical advances are made possible by local precipitation and amplification of insoluble colour dyes inside the hydrogel networks. For the first time, enzymatic accumulation of colour dyes in hydrogel particles is reported and the kinetics of colour development is characterized in this work. By taking advantage of the colour signals in the visible spectrum, the hydrogel microparticles were imaged and analysed using low-cost portable devices. The colorimetric multiplex immunoassay was used to successfully detect three target biomarkers of preeclampsia and validated clinically using healthy and patient-derived plasma samples.

中文翻译：

水凝胶颗粒中基于沉淀的比色多重免疫测定。

尽管对可访问的诊断技术的需求不断增长，但当前的方法仍难以以可接受的灵敏度和便携性同时检测多种分析物。比色测定法由于其信号读取的简单性而被广泛使用，但是缺乏多重性一直是永久的限制。同时，基于颗粒的测定法通过为每种分析物分配一个身份代码来提供多重检测，但它们通常需要不适合便携式诊断的基于实验室的设备。在这里，通过两种方法的结合，本文报道了一种基于水凝胶微粒的比色多重免疫分析方法，该方法实现了两全其美。与基于实验室的酶联免疫吸附测定（ELISA）相比，低成本便携式多重测定具有更高的灵敏度和更大的动态范围。通过在水凝胶网络内部局部沉淀和溶解不溶性染料，这些关键的进展成为可能。首次报道了染料在水凝胶颗粒中的酶促积累，并且在这项工作中表征了显色动力学。通过利用可见光谱中的彩色信号，使用低成本便携式设备对水凝胶微粒进行成像和分析。比色法多重免疫测定法可成功检测先兆子痫的三种靶标生物标志物，并使用健康和患者来源的血浆样品进行临床验证。报道了染料在水凝胶颗粒中的酶促积累，并且在这项工作中表征了显色动力学。通过利用可见光谱中的彩色信号，使用低成本便携式设备对水凝胶微粒进行成像和分析。比色法多重免疫测定法可成功检测先兆子痫的三种靶标生物标志物，并使用健康和患者来源的血浆样品进行临床验证。报道了染料在水凝胶颗粒中的酶促积累，并且在这项工作中表征了显色动力学。通过利用可见光谱中的彩色信号，使用低成本便携式设备对水凝胶微粒进行成像和分析。比色多重免疫测定法被用来成功检测先兆子痫的三种靶标生物标志物，并使用健康和患者来源的血浆样品进行了临床验证。

更新日期：2020-08-11

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