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The impact of sample preparation on the elemental composition of soft tissues assessed by laser ablation ICP-MS
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-06-23 , DOI: 10.1039/d0ja00115e Agata Jagielska 1, 2, 3, 4, 5 , Anna Ruszczyńska 1, 2, 3, 4, 5 , Ewa Bulska 1, 2, 3, 4, 5 , Barbara Wagner 1, 2, 3, 4, 5
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-06-23 , DOI: 10.1039/d0ja00115e Agata Jagielska 1, 2, 3, 4, 5 , Anna Ruszczyńska 1, 2, 3, 4, 5 , Ewa Bulska 1, 2, 3, 4, 5 , Barbara Wagner 1, 2, 3, 4, 5
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Selected procedures of soft biological sample stabilization for analytical and medical diagnosis purposes (oven drying, short freeze drying and long freeze drying) were compared in terms of their suitability for elemental analysis by means of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The study was performed on model samples (chicken liver). Biologically important elements, such as Na, Mg, P, S, Cl, K, Ca, Mn, Fe, Cu, Zn and Sr, were taken into account and subjected to statistical analysis (Single Factor Analysis of Variance and Duncan's post hoc-test). It was found out that freeze drying (but not oven drying) reduced the content of the elements Mg, Ca, Mn, Cu, Zn, Sr, P, and S; while the content of typical blood electrolytes (Na, Cl, and K) enhanced in the same samples. The evaluation of sample preparation processes was performed for ICP-MS bulk analysis of tissues, prepared with and without sample homogenization. Significant loss of Mn, Cu and Zn after freeze drying was confirmed while no effect (or a barely opposite effect compared to LA-ICP-MS analysis) was observed for blood electrolytes (Na and K), which indicates specific surface enrichment. Based on the results described in this work it was established that any water removal may induce changes in the primary elemental composition of soft tissues even if it allows their physical stabilization.
中文翻译：
通过激光烧蚀ICP-MS评估样品制备对软组织元素组成的影响
比较了用于分析和医学诊断目的的选定的软生物样品稳定程序（烤箱干燥，短期冷冻干燥和长时间冷冻干燥），比较了它们适合通过激光烧蚀电感耦合等离子体质谱法（LA-ICP-多发性硬化症）。该研究是在模型样本（鸡肝）上进行的。将生物学上重要的元素（例如Na，Mg，P，S，Cl，K，Ca，Mn，Fe，Cu，Zn和Sr）考虑在内并进行统计分析（方差和邓肯事后分析的单因素分析）-测试）。发现冷冻干燥（而不是烘箱干燥）降低了元素Mg，Ca，Mn，Cu，Zn，Sr，P和S的含量。而相同样品中典型血液电解质（Na，Cl和K）的含量增加。进行样品制备过程的评估，以对组织进行ICP-MS批量分析（有或没有样品均质化）。确认冷冻干燥后Mn，Cu和Zn大量损失，而血液电解质（Na和K）未观察到任何影响（或与LA-ICP-MS分析相比几乎相反的影响），这表明比表面积富集。根据这项工作中描述的结果，可以确定，除水会导致软组织的物理稳定，因此可能会导致软组织的主要元素组成发生变化。
更新日期：2020-07-08
中文翻译：
通过激光烧蚀ICP-MS评估样品制备对软组织元素组成的影响
比较了用于分析和医学诊断目的的选定的软生物样品稳定程序（烤箱干燥，短期冷冻干燥和长时间冷冻干燥），比较了它们适合通过激光烧蚀电感耦合等离子体质谱法（LA-ICP-多发性硬化症）。该研究是在模型样本（鸡肝）上进行的。将生物学上重要的元素（例如Na，Mg，P，S，Cl，K，Ca，Mn，Fe，Cu，Zn和Sr）考虑在内并进行统计分析（方差和邓肯事后分析的单因素分析）-测试）。发现冷冻干燥（而不是烘箱干燥）降低了元素Mg，Ca，Mn，Cu，Zn，Sr，P和S的含量。而相同样品中典型血液电解质（Na，Cl和K）的含量增加。进行样品制备过程的评估，以对组织进行ICP-MS批量分析（有或没有样品均质化）。确认冷冻干燥后Mn，Cu和Zn大量损失，而血液电解质（Na和K）未观察到任何影响（或与LA-ICP-MS分析相比几乎相反的影响），这表明比表面积富集。根据这项工作中描述的结果，可以确定，除水会导致软组织的物理稳定，因此可能会导致软组织的主要元素组成发生变化。
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