With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems. For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol–gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine. The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed. Repetitive peaks from acids along with stretching confirmed the chemical interactions of the composites. Swelling ability, porosity, and in vitro degradation and biomineralization were analysed for up to 7 days. The results indicated that reduced swelling and degradation enhanced apatite formation. Hemocompatibility and the hemostatic properties on scaffolds were also systematically investigated. Additionally, significant cyto-compatibility and proliferation were noted in a culture with KB3-1. Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest. Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.

中文翻译：

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生物活性，可降解和多功能的生物玻璃和藻酸盐复合材料的三维膜状支架，用于组织再生应用。

随着世界范围内老年人口和相关老年疾病的增加，对退化器官系统的再生有极大的需求。为此，通过溶胶-凝胶辅助冷冻干燥法制备了生物活性玻璃颗粒（nBG）集成藻酸盐（Alg）复合膜支架，并验证了其在再生医学中的多功能性。证实了nBG和Alg无定形宽峰的辉绿色高结晶结构的存在。来自酸的重复峰以及拉伸证实了复合材料的化学相互作用。溶胀能力，孔隙率和体外降解和生物矿化分析长达7天。结果表明，减少的溶胀和降解增强了磷灰石的形成。还对支架的血液相容性和止血性能进行了系统研究。另外，在具有KB3-1的培养物中注意到显着的细胞相容性和增殖。与HDF细胞和KB3-1细胞进行的其他3-D共培养在Alg，nBG / Alg和nBG-Zr / Alg上显示出球状形成，并具有确定目标类器官所需的强大动力。因此，结果表明这些3D水凝胶膜在再生医学领域可能提供无限的可能性，特别是作为支持骨骼再生，血管内形成和新组织形成（例如软骨和韧带）的细胞外基质（ECM） 。