Hypoxia imaging in living cells, tissues and zebrafish with a nitroreductase-specific fluorescent probe.,Analyst

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Hypoxia imaging in living cells, tissues and zebrafish with a nitroreductase-specific fluorescent probe.
Analyst ( IF 3.6 ) Pub Date : 2020-06-23 , DOI: 10.1039/d0an00378f
Li Fan ₁ , Qi Zan , Bo Lin , Xiaodong Wang , Xiaojuan Gong , Zhonghua Zhao , Shaomin Shuang , Chuan Dong , Man Shing Wong

Affiliation

Hypoxia in solid tumors is directly linked to the elevated levels of endogenous nitroreductase (NTR). We present a novel fluorescent probe, namely NTNO, for nitroreductase-specific detection based on the NTR-catalyzed reduction of the nitro unit to an amine functionality, and demonstrated its application for hypoxia imaging. NTNO was designed by incorporating a nitro unit as the NTR response site into a benzothiazole derivative. Upon reacting with NTR in the presence of reduced nicotinamide adenine dinucleotide (NADH), the fluorescence of the probe was strongly and sensitively turned on, with a good linearity in the NTR concentration range of 0.5–8.0 μg mL⁻¹ and a detection limit of 48 ng mL⁻¹. Most notably, NTNO has been successfully used for imaging hypoxia levels in living cells, tumor tissues and zebrafish, making it of great potential to monitor NTR in biological systems.

中文翻译：

使用硝基还原酶特异性荧光探针在活细胞，组织和斑马鱼中进行低氧成像。

实体瘤中的缺氧与内源性硝基还原酶（NTR）水平升高直接相关。我们提出了一种新型的荧光探针，即NTNO，用于基于NTR催化的硝基单元还原成胺官能团的硝基还原酶特异性检测，并证明了其在低氧成像中的应用。通过将硝基单元作为NTR响应位点并入苯并噻唑衍生物来设计NTNO。在还原性烟酰胺腺嘌呤二核苷酸（NADH）存在下与NTR反应时，探针的荧光强烈而灵敏地开启，在NTR浓度范围为0.5–8.0μgmL ^-1时线性良好，检出限为48 ng毫升^-1。最值得注意的是，NTNO 已成功地用于活细胞，肿瘤组织和斑马鱼中低氧水平的成像，使其具有监测生物系统中NTR的巨大潜力。

更新日期：2020-08-10

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