Background. Both antioxidant and prooxidant activities have been previously reported for cerium oxide (CeO₂). The aim of this study was to investigate the effects of CeO₂ at different doses on changes in kidney tissues and markers in neonatal mice. Methods. We randomly divided 30 pregnant NMRI mice into five groups ( per group)—a control group and four groups treated with intraperitoneal (i.p.) administration of different doses of CeO₂ (10, 25, 80, or 250 mg/kg body weight (bw)) on gestation days (GD) 7 and GD14. At the end of the treatment period, we analyzed the kidney tissues and serum samples. The levels of two serum redox markers, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP), were determined. Data were analyzed using one-way ANOVA and Tukey’s test, and a value of <0.05 was considered significant. Results. The mean total volumes of the renal corpuscle, glomeruli, and Bowman’s capsule membranes significantly increased, and there was a significant decrease in the mean total volume of Bowman’s space in the high-dose CeO₂ group compared to that in the control group. No statistically significant differences existed in the serum levels of MDA and FRAP in the treated and control groups. Conclusion. Our results suggest that high doses of CeO₂ impair fetal renal development in pregnant mice, which results in kidney damage. Therefore, CeO₂ administration during pregnancy could have dose-dependent adverse effects on the developing kidneys in neonates.

中文翻译：

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妊娠期氧化铈对新生儿 NMRI 小鼠肾脏组织毒性作用的立体学研究。

背景。先前已经报道了氧化铈（CeO ₂）的抗氧化和促氧化活性。本研究旨在探讨不同剂量CeO ₂对新生小鼠肾组织和标志物变化的影响。方法。我们将 30 只怀孕的 NMRI 小鼠随机分为 5 组（每组）——对照组和四组在妊娠天（GD）7和GD14腹膜内（ip）给药不同剂量的CeO ₂（10、25、80或250mg/kg体重（bw）） . 在治疗期结束时，我们分析了肾组织和血清样本。测定了两种血清氧化还原标志物丙二醛 (MDA) 和铁还原/抗氧化能力 (FRAP) 的水平。使用单因素方差分析和 Tukey 检验分析数据，<0.05 的值被认为是显着的。结果。_{高剂量CeO 2}肾小体、肾小球和Bowman囊膜的平均总体积显着增加，Bowman空间的平均总体积显着减少组与对照组相比。治疗组和对照组血清MDA和FRAP水平差异无统计学意义。结论。我们的研究结果表明，高剂量的 CeO ₂会损害怀孕小鼠的胎儿肾脏发育，从而导致肾脏损伤。因此，怀孕期间给予CeO ₂可能对新生儿的发育中的肾脏产生剂量依赖性的副作用。