Abstract

In vitro and in vivo studies were conducted to examine the effect of silkworm pupae oil on methane (CH₄) emission and methanogens diversity. Five graded levels (2, 4, 6, 8 and 10%) of silkworm pupae oil were tested in vitro. Eighteen Mandya adult sheep were divided into three groups. All the animals were fed on similar basal diet except the oil supplementation in test groups. Oil level for supplementation was decided on the basis of in vitro study. In vitro study indicated a reduction of 22% in CH₄ production with 2% oil supplementation. Animals in test groups were supplemented with oil (2%) either daily (CON) or intermittently (INT) on every alternate week for all the seven days. A significant reduction of 17–20% in enteric CH₄ emission (g/d) was achieved due to oil supplementation in sheep. However, No variation was established between test groups CON and INT. In present study, Methanobrevibacter was major genus contributed ∼90% of the total rumen methanogens; whilst Methanobrevibacter gottschalkii was the most abundant methanogens species. Abundance of Methanobrevibacter ruminantium was affected with the oil supplementation. It can be concluded that the silkworm pupae oil at 2% can decrease CH₄ emission by 15–20%.

摘要

进行了体外和体内研究，以检查蚕蛹油对甲烷 (CH ₄ ) 排放和产甲烷菌多样性的影响。在体外测试了蚕蛹油的五个等级（2、4、6、8 和 10%）。十八只满地亚成年羊被分成三组。除了在测试组中补充油外，所有动物都以相似的基础饮食喂养。补充油量是根据体外研究确定的。体外研究表明 CH ₄减少了 22%生产时添加 2% 的油。测试组中的动物在所有 7 天内每隔一周每天 (CON) 或间歇性 (INT) 补充油 (2%)。由于绵羊补充油，肠道 CH ₄排放量 (g/d)显着降低了 17-20% 。然而，在测试组 CON 和 INT 之间没有建立差异。在本研究中，甲烷短杆菌是瘤胃产甲烷菌的主要属，占瘤胃产甲烷菌总量的 90%；而Methanobrevibacter gottschalkii是最丰富的产甲烷菌物种。补充油会影响反刍甲烷短杆菌的丰度。可以得出结论，2%的蚕蛹油可以降低CH ₄ 排放量减少 15–20%。