ABSTRACT

Background

Ischemic stroke is a clinical condition characterized by focal or global cerebral dysfunction resulting from inhibition of brain blood flow. Genetic factors play an important role in the pathogenesis of ischemic stroke. As a result of IL-18 (−607 C/A, −137 G/C) gene variations, it is thought that binding of transcription factors may be affected and IL-18 mRNA expression can be modulated. Therefore, the purpose of our study is to investigate the roles of IL-18 (−607 C/A), IL-18 (−137 G/C) gene variations in the development of ischemic stroke in Trakya Region of Turkey.

Methods

Our study was performed with 90 ischemic stroke patients and 89 healthy controls. Genotype distributions of IL-18 (−607 C/A, −137 G/C) gene variations were determined using polymerase chain reaction (PCR) method.

Results

GC genotype and CA genotype of IL-18 (−137 G/C) and IL-18 (−607 C/A) gene variations were determined higher significantly in patent group as compared with other genotypes. However, the statistically significant difference was not determined between patients with ischemic stroke and healthy control groups in terms of IL-18 (−137 G/C) and IL-18 (−607 C/A) gene variations (p > 0,05). Allele frequencies of IL-18 (−137 G/C) and IL-18 (−607 C/A) in patient and control groups were significantly different from the Hardy-Weinberg distribution (p < .001 for all).

Conclusion

Although these gene variations’ genotype distributions were not determined as a genetic risk factor for the development of ischemic stroke, allele frequencies of IL-18 (−137 G/C) and IL-18 (−607 C/A) in patient and control groups were significantly different from the Hardy-Weinberg distribution.

中文翻译：

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土耳其色雷斯地区IL-18 (-607 C/A)、IL-18 (-137 G/C)基因变异与缺血性脑卒中发病关系的调查

摘要

背景

缺血性中风是一种临床病症，其特征在于由于脑血流受抑制而导致的局灶性或全脑功能障碍。遗传因素在缺血性脑卒中的发病机制中起重要作用。由于 IL-18 (-607 C/A, -137 G/C) 基因变异，人们认为转录因子的结合可能会受到影响，并且可以调节 IL-18 mRNA 的表达。因此，我们研究的目的是研究 IL-18 (-607 C/A)、IL-18 (-137 G/C) 基因变异在土耳其 Trakya 地区缺血性中风发展中的作用。

方法

我们的研究是对 90 名缺血性卒中患者和 89 名健康对照进行的。使用聚合酶链反应 (PCR) 方法确定 IL-18 (-607 C/A, -137 G/C) 基因变异的基因型分布。

结果

与其他基因型相比，专利组中IL-18（-137 G/C）和IL-18（-607 C/A）基因变异的GC基因型和CA基因型显着更高。然而，就 IL-18 (-137 G/C) 和 IL-18 (-607 C/A) 基因变异而言，缺血性卒中患者与健康对照组之间未确定统计学显着差异 ( p > 0,05 ）。患者和对照组中 IL-18 (-137 G/C) 和 IL-18 (-607 C/A) 的等位基因频率与 Hardy-Weinberg 分布显着不同（所有p < .001）。

结论

尽管这些基因变异的基因型分布未被确定为缺血性中风发展的遗传风险因素，但患者和对照中 IL-18 (-137 G/C) 和 IL-18 (-607 C/A) 的等位基因频率组与 Hardy-Weinberg 分布有显着差异。