Abstract For several years in Europe, there has been a growing interest in the use of native sources of protein (e.g. lupin seeds) in poultry nutrition. The current study aimed to investigate the effectiveness of a microbial phytase in broiler diets with the addition of different levels of white lupin seeds. A total of 480 one-day-old male broiler chickens (Ross 308) were randomly divided into six dietary treatments (10 replications/8 birds per group). The basal diet contained SBM as the main protein source and experimental treatments were prepared with white lupin meal (WLM) at 3 levels (0, 10, and 20%) and with or without phytase inclusion. The experiment was divided into two feeding periods: from 1 to 14 days (starter) and from 15 to 35 days (grower). Diets with phytase addition were deficient in Ca and non-phytate P. All diets were fed in mash form and offered ad libitum. On day 35 excreta were collected and on day 36, ten chickens from each group were euthanized and blood, tibia, and digesta samples were collected for further analysis. The 20% addition of WLM negatively increased the content of phytic-P. The results showed that feed conversion ratio (FCR) and body weight gain (BWG) were not affected by phytase inclusion but by the WLM level alone. In addition, birds fed the diet with 20% WLM were characterized by having the lowest BWG and the highest FCR of all groups. There were no significant differences in feed intake (FI) among chickens fed control and experimental treatments. Regardless of the white lupin level addition, phytase addition improved (P<0.001) nitrogen-corrected apparent metabolizable energy (AMEN). In conclusion, the addition of phytase positively influenced the performance and availability of minerals (Ca and P) regardless of the level of WLM used. However, with regard to the use of WLM in poultry nutrition, it can be assumed that 10% addition is safe and does not affect performance.

中文翻译：

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微生物植酸酶改善了以豆粕和白羽扇豆（Lupinus albus）粉为基础饲喂的肉鸡的性能和骨骼特性

摘要 几年来，在欧洲，在家禽营养中使用天然蛋白质来源（例如羽扇豆种子）越来越受到关注。目前的研究旨在调查一种微生物植酸酶在肉鸡日粮中添加不同水平的白羽扇豆种子的有效性。总共 480 只 1 日龄雄性肉鸡 (Ross 308) 被随机分成六个饮食处理（每组 8 只鸡 10 次重复）。基础日粮含有 SBM 作为主要蛋白质来源，实验处理是用白羽扇豆粉 (WLM) 在 3 个水平 (0、10 和 20%) 和含有或不含植酸酶的情况下制备的。实验分为两个饲养期：1 到 14 天（开始）和 15 到 35 天（生长）。添加植酸酶的日粮缺乏钙和非植酸磷。所有饮食均以醪液形式喂养并随意提供。在第 35 天收集排泄物，在第 36 天，对每组的 10 只鸡实施安乐死，并收集血液、胫骨和食糜样品用于进一步分析。添加 20% 的 WLM 会负增加植酸磷的含量。结果表明，饲料转化率 (FCR) 和体重增加 (BWG) 不受添加植酸酶的影响，而仅受 WLM 水平的影响。此外，饲喂含 20% WLM 的日粮的鸡的特征在于所有组中的 BWG 最低和 FCR 最高。饲喂对照和实验处理的鸡之间的采食量（FI）没有显着差异。不管白羽扇豆添加水平如何，添加植酸酶都提高了（P<0.001）氮校正表观代谢能（AMEN）。综上所述，无论使用的 WLM 水平如何，添加植酸酶都会对矿物质（Ca 和 P）的性能和可用性产生积极影响。但是，关于在家禽营养中使用 WLM，可以假设添加 10% 是安全的并且不会影响性能。