Abstract In this study, the basal diet was supplemented with ethanolic extract of purslane (Portulaca oleracea L.) and the possible effects on growth performance, anti-oxidative, and immune activities of grass carp were evaluated. Fish with initial weight 1.23 ± 0.11 g were randomly divided into four groups (triplicates) and fed purslane extract at 0% (T0), 0.5% (T1), 1% (T2), and 1.5% (T3) for 56 days. At the end of the feeding trial, the results showed that growth parameters were enhanced in T1 groups compared to the control group (P<0.05). Lipase activity in T1 and T2 groups increased, whereas no significant changes were noticed in cases of amylase and protease activities (P>0.05). Catalase and superoxide dismutase activities were enhanced in all groups fed the supplemented diets in comparison with the control group (P<0.05). However, no significant alteration was noticed in the case of glutathione peroxidase activity following the administration of purslane extract (P>0.05). A significant increase in total immunoglobulin level was noted in the T1 group, but lysozyme activity was higher in T1 and T2 groups compared to the control group (P<0.05). In conclusion, supplementation of grass carp diet with the purslane ethanolic extract, especially at 0.5%, can improve growth performance, lipase activity, the antioxidant enzyme activities as well as the immune response of grass carp fingerlings.

中文翻译：

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评估马齿苋 (Portulaca oleracea L.) 对草鱼 (Ctenopharyngodon idella) 生长性能、抗氧化和免疫活性的影响

摘要 本研究在基础日粮中添加马齿苋（Portulaca oleracea L.）乙醇提取物，评估其对草鱼生长性能、抗氧化和免疫活性的可能影响。将初始体重 1.23 ± 0.11 克的鱼随机分成四组（一式三份），并以 0% (T0)、0.5% (T1)、1% (T2) 和 1.5% (T3) 喂食马齿苋提取物 56 天。饲养试验结束时，结果显示T1组生长参数较对照组增强（P<0.05）。T1和T2组脂肪酶活性增加，而淀粉酶和蛋白酶活性无明显变化（P>0.05）。与对照组相比，饲喂补充饮食的所有组的过氧化氢酶和超氧化物歧化酶活性均增强（P<0.05）。然而，马齿苋提取物给药后谷胱甘肽过氧化物酶活性未见明显变化（P>0.05）。T1组总免疫球蛋白水平显着增加，但与对照组相比，T1和T2组溶菌酶活性更高（P<0.05）。总之，在草鱼饲料中添加马齿苋乙醇提取物，尤其是 0.5%，可以提高草鱼鱼种的生长性能、脂肪酶活性、抗氧化酶活性以及免疫反应。但溶菌酶活性在T1和T2组高于对照组（P<0.05）。总之，在草鱼饲料中添加马齿苋乙醇提取物，尤其是 0.5%，可以提高草鱼鱼种的生长性能、脂肪酶活性、抗氧化酶活性以及免疫反应。但溶菌酶活性在T1和T2组高于对照组（P<0.05）。总之，在草鱼饲料中添加马齿苋乙醇提取物，尤其是 0.5%，可以提高草鱼鱼种的生长性能、脂肪酶活性、抗氧化酶活性以及免疫反应。