Puzzle-shaped pavement cells provide a powerful model system to investigate the cellular and subcellular processes underlying complex cell-shape determination in plants. To better understand pavement cell-shape acquisition and the role of auxin in this process, we focused on the spirals of young stomatal lineage ground cells of Arabidopsis leaf epidermis. The predictability of lobe formation in these cells allowed us to demonstrate that the auxin response gradient forms within the cells of the spiral and fluctuates based on the particular stage of lobe development. We revealed that specific localization of auxin transporters at the different membranes of these young cells changes during the course of lobe formation, suggesting that these fluctuating auxin response gradients are orchestrated via auxin transport to control lobe formation and determine pavement cell shape.

拼图形状的铺面细胞提供了一个强大的模型系统，可以研究植物中复杂的细胞形状确定背后的细胞和亚细胞过程。为了更好地了解路面细胞的形状获取和生长素在该过程中的作用，我们集中在拟南芥气孔世代地面细胞的年轻螺旋上叶表皮。这些细胞中叶形成的可预测性使我们能够证明生长素应答梯度在螺旋细胞内形成并根据叶发育的特定阶段而波动。我们揭示了，在叶片形成过程中，这些年轻细胞的不同膜上生长素转运蛋白的特定定位发生了变化，这表明这些波动的生长素反应梯度是通过生长素转运来编排的，以控制叶片的形成并确定路面细胞的形状。