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AtHsc70-1 negatively regulates the basal heat tolerance in Arabidopsis thaliana through affecting the activity of HsfAs and Hsp101.
The Plant Journal ( IF 6.2 ) Pub Date : 2020-06-23 , DOI: 10.1111/tpj.14883 Lalit D Tiwari 1 , Lisha Khungar 1 , Anil Grover 1
The Plant Journal ( IF 6.2 ) Pub Date : 2020-06-23 , DOI: 10.1111/tpj.14883 Lalit D Tiwari 1 , Lisha Khungar 1 , Anil Grover 1
Affiliation
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Heat shock protein 70 (Hsp70) chaperones are highly conserved and essential proteins with diverse cellular functions, including plant abiotic stress tolerance. Hsp70 proteins have been linked with basal heat tolerance in plants. Hsp101 likewise is an important chaperone protein that plays a critical role in heat tolerance in plants. We observed that Arabidopsis hsc70‐1 mutant seedlings show elevated basal heat tolerance compared with wild‐type. Over‐expression of Hsc70‐1 resulted in increased heat sensitivity. Hsp101 transcript and protein levels were increased during non‐heat stress (HS) and post‐HS conditions in hsc70‐1 mutant seedlings. In contrast, Hsp101 was repressed in Hsc70‐1 over‐expressing plants after post‐HS conditions. Hsc70‐1 showed physical interaction with HsfA1d and HsfA1e protein in the cytosol under non‐HS conditions. In transient reporter gene analysis, HsfA1d, HsfA1e and HsfA2 showed transcriptional response on the Hsp101 promoter. HsfA1d and HsfA2 transcripts were at higher levels in hsc70‐1 mutant compared with wild‐type. We provide genetic evidence that Hsc70‐1 is a negative regulator affecting HsfA1d/A1e/A2 activators, which in turn regulate Hsp101 expression and basal thermotolerance.
中文翻译：
AtHsc70-1通过影响HsfAs和Hsp101的活性，对拟南芥的基础耐热性产生负调控。
热休克蛋白70（Hsp70）分子伴侣是高度保守的必需蛋白，具有多种细胞功能，包括植物非生物胁迫耐受性。Hsp70蛋白与植物的基础耐热性有关。Hsp101同样是重要的伴侣蛋白，在植物的耐热性中起关键作用。我们观察到，与野生型相比，拟南芥hsc70-1突变苗显示出更高的基础耐热性。Hsc70-1的过表达导致热敏感性增加。在hsc70-1突变苗中，在非热胁迫（HS）和HS后的条件下，Hsp101转录和蛋白质水平均升高。相反，Hsp101HS后条件在Hsc70-1过表达植物中被抑制。在非HS条件下，Hsc70-1与胞质溶胶中的HsfA1d和HsfA1e蛋白发生物理相互作用。在瞬时报告基因分析中，HsfA1d，HsfA1e和HsfA2在Hsp101启动子上显示出转录反应。与野生型相比，hsc70-1突变体中的HsfA1d和HsfA2转录本较高。我们提供了遗传证据，证明Hsc70-1是影响HsfA1d / A1e / A2激活剂的负调节剂，进而调节Hsp101的表达和基础耐热性。
更新日期：2020-06-23
中文翻译：
AtHsc70-1通过影响HsfAs和Hsp101的活性，对拟南芥的基础耐热性产生负调控。
热休克蛋白70（Hsp70）分子伴侣是高度保守的必需蛋白，具有多种细胞功能，包括植物非生物胁迫耐受性。Hsp70蛋白与植物的基础耐热性有关。Hsp101同样是重要的伴侣蛋白，在植物的耐热性中起关键作用。我们观察到，与野生型相比，拟南芥hsc70-1突变苗显示出更高的基础耐热性。Hsc70-1的过表达导致热敏感性增加。在hsc70-1突变苗中，在非热胁迫（HS）和HS后的条件下，Hsp101转录和蛋白质水平均升高。相反，Hsp101HS后条件在Hsc70-1过表达植物中被抑制。在非HS条件下，Hsc70-1与胞质溶胶中的HsfA1d和HsfA1e蛋白发生物理相互作用。在瞬时报告基因分析中，HsfA1d，HsfA1e和HsfA2在Hsp101启动子上显示出转录反应。与野生型相比，hsc70-1突变体中的HsfA1d和HsfA2转录本较高。我们提供了遗传证据，证明Hsc70-1是影响HsfA1d / A1e / A2激活剂的负调节剂，进而调节Hsp101的表达和基础耐热性。
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