The information about the impact of copper pipes on the growth of Legionella pneumophila in premise plumbing is controversial. For this reason, pipe segments of copper, stainless steel (SS), mild steel (MS), polyethylene, chlorinated polyvinylchloride (CPVC) and glass (controls) were exposed to intermittently flowing (20 min stagnation time) nonchlorinated tap water of 37 °C or 16 °C (ambient temperature) during six months to study the impact of metals on biofilm formation and growth of L. pneumophila. Biofilm concentrations (BfC, measured as ATP) on copper were 3 (at 37 °C) to 6 (at 16 °C) times higher than on SS. The maximum colony counts of L. pneumophila on the materials tested at 37 °C showed a quadratic relationship with the associated BfCs, with highest values on copper and MS. The average Cu concentration on the glass control of copper (glass-copper) was more than two log units lower than the Fe concentration on glass-MS, suggesting that copper released less corrosion by-products than MS. The release of corrosion by-products with attached biomass from MS most likely enhanced biofilm formation on glass-MS. Cloning and 16S RNA gene sequence analysis of the predominating biofilm bacteria revealed that an uncultured Xanthobacteraceae bacterium and Reyranella accounted for 75% of the bacterial community on copper at 37 °C. The nitrite-oxidizing Nitrospira moscoviensis, which can also utilize hydrogen (H₂) and formate, accounted for >50% of the bacterial abundance in the biofilms on MS and glass-MS at 37 °C. The predominating presence of the strictly anaerobic non-fermentative Fe(III)-reducing Geobacter and the Fe(II)-oxidizing Gallionella on MS exposed to tap water of 16 °C indicated anoxic niches and the availability of H₂, low molecular weight carboxylic acids (LMWCAs) and Fe(II) at the MS surface. LMWCAs likely also promoted bacterial growth on copper, but the release mechanisms from natural organic matter at the surface of corroding metals are unclear. The effects of water stagnation time and flow dynamics on biofilm formation on copper requires further investigation.

有关在室内管道中铜管对嗜肺军团菌生长的影响的信息引起争议。因此，将铜，不锈钢（SS），低碳钢（MS），聚乙烯，氯化聚氯乙烯（CPVC）和玻璃（对照）的管段暴露于间歇性流动（停滞时间20分钟）37°C的非氯化自来水中在六个月内的摄氏温度（摄氏16度）或摄氏16度（环境温度）下，研究金属对嗜肺乳杆菌生物膜形成和生长的影响。铜上的生物膜浓度（BfC，以ATP衡量）比SS高3倍（在37°C时）至6倍（在16°C时）。嗜肺乳杆菌的最大菌落数在37°C下测试的材料中的BfCs与相关的BfCs呈二次关系，在铜和MS上最高。铜（玻璃-铜）的玻璃对照上的平均Cu浓度比玻璃-MS上的Fe浓度低两个对数单位以上，这表明铜释放的腐蚀副产物少于MS。MS释放附着有生物质的腐蚀副产物，最有可能增强了玻璃MS上生物膜的形成。对主要生物膜细菌的克隆和16S RNA基因序列分析表明，在37°C时，未培养的Xanthobacteraceae细菌和Reyranella占铜细菌群落的75％。亚硝酸氧化的硝化螺旋藻（Motroviensis），也可以利用氢（H₂）和甲酸盐，占37°C下MS和玻璃-MS上生物膜中细菌丰度的50％以上。-还原所述严格厌氧非发酵的Fe（III）的存在为主的地杆菌和铁（II）氧化嘉利翁氏菌在MS暴露于16℃下所指示缺氧壁龛和H的可用性的自来水₂，低分子量羧酸MS表面的酸（LMWCAs）和Fe（II）。LMWCAs可能还促进了细菌在铜上的生长，但是尚不清楚腐蚀金属表面天然有机物的释放机理。水停滞时间和流动动力学对铜上生物膜形成的影响需要进一步研究。