Abstract The main objective of our study was to illuminate effects of sodium selenite (Na2SeO3) on growth, metabolism and enzyme activities of Cordyceps militaris. By adding Na2SeO3 in fermentation medium (7 mg/L), it was found that mycelium of C. militaris was stronger with more plump spores and its biomass was higher accompanied by the maximum (13.19 g/L) at 6 d of culture. Besides, Na2SeO3 also caused the enhancement of total thiol (T-SH), non-protein thiol (NP-SH) contents and the biosynthesis of Se-polysaccharide (Se-CMP) with discriminatory molecular weight, optical rotation, UV–vis and FT-IR spectra. Activities of antioxidase including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and enzymes involved in polysaccharide synthesis including phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), UDPG-pyrophosphorylase (UGP) enhanced to different extent. Their corresponding genes were also up-regulated but cat gene (encoding CAT). Se-enrichment of C. militaris provided a good way for desirable biomass and polysaccharide biosynthesis in further research.

中文翻译：

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Na2SeO3对蛹虫草生长、代谢、抗氧化酶及多糖合成酶的影响

摘要 我们研究的主要目的是阐明亚硒酸钠 (Na2SeO3) 对蛹虫草生长、代谢和酶活性的影响。在发酵培养基中加入Na2SeO3（7mg/L），发现蛹虫草菌丝体更强，孢子更丰满，生物量更高，培养6d时达到最大值（13.19g/L）。此外，Na2SeO3 还引起总硫醇 (T-SH)、非蛋白硫醇 (NP-SH) 含量的增加和硒多糖 (Se-CMP) 的生物合成，具有区分性分子量、旋光度、UV-vis 和FT-IR 光谱。抗氧化酶的活性，包括过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-PX) 和参与多糖合成的酶，包括磷酸葡萄糖变位酶 (PGM)、磷酸葡萄糖异构酶 (PGI)、UDPG-焦磷酸化酶（UGP）有不同程度的增强。它们的相应基因也被上调，但是 cat 基因（编码 CAT）。C. militaris 富硒为进一步研究所需的生物质和多糖生物合成提供了很好的途径。