The rapid development of high-throughput sequencing techniques has led biology into the big-data era. Data analyses using various bioinformatics tools rely on programming and command-line environments, which are challenging and time-consuming for most wet-lab biologists. Here, we present TBtools (a Toolkit for Biologists integrating various biological data-handling tools), a stand-alone software with a user-friendly interface. The toolkit incorporates over 130 functions, which are designed to meet the increasing demand for big-data analyses, ranging from bulk sequence processing to interactive data visualization. A wide variety of graphs can be prepared in TBtools using a new plotting engine (“JIGplot”) developed to maximize their interactive ability; this engine allows quick point-and-click modification of almost every graphic feature. TBtools is platform-independent software that can be run under all operating systems with Java Runtime Environment 1.6 or newer. It is freely available to non-commercial users at https://github.com/CJ-Chen/TBtools/releases.

高通量测序技术的快速发展已将生物学带入了大数据时代。使用各种生物信息学工具进行的数据分析依赖于编程和命令行环境，这对于大多数湿实验室生物学家而言既具有挑战性又耗时。在这里，我们目前TBtools（一个牛逼的oolkit乙iologists整合各种生物数据处理工具），具有用户友好界面的独立软件。该工具包包含130多种功能，旨在满足对大数据分析不断增长的需求，从批量序列处理到交互式数据可视化。可以使用新开发的绘图引擎（“ JIGplot”）在TBtools中准备各种图形，以最大化其交互能力；该引擎允许快速点击修改几乎所有图形功能。TBtools是与平台无关的软件，可以在具有Java Runtime Environment 1.6或更高版本的所有操作系统上运行。非商业用户可以从https://github.com/CJ-Chen/TBtools/releases免费获得。